Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
βA4-crystallin CRISPR/Cas9 KO Plasmid (m): sc-419824
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- βA4-crystallin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the βA4-crystallin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
βA4-crystallin CRISPR/Cas9 KO Plasmid (m) | sc-419824 | 20 µg | $397.00 |
Overview
Cryba4 encodes βA4-crystallin, a major structural crystallin of the mouse ocular lens that contributes to refractive properties and long-term lens transparency. As a member of the β/γ-crystallin superfamily, βA4-crystallin participates in lens fiber cell differentiation and formation of tightly packed, highly ordered protein assemblies that maintain optical homogeneity. Its stability and interactions within crystallin networks influence protein homeostasis processes such as chaperone-assisted folding and protection from aggregation under oxidative and aging-associated stress. Disruption or dysregulation of crystallin composition is linked to lens opacification phenotypes, supporting Cryba4 as a relevant locus for cataract-related mechanisms and ocular development studies.
βA4-crystallin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cryba4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cryba4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cryba4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish βA4-crystallin protein expression.
This CRISPR knockout system enables efficient generation of Cryba4-deficient cell models for investigation of βA4-crystallin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cryba4 exon(s) critical for βA4-crystallin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cryba4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by βA4-crystallin CRISPR/Cas9 KO Plasmid (m) and βA4-crystallin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cryba4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by βA4-crystallin HDR Plasmid (m) and βA4-crystallin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cryba4 homology arms to support homology-directed repair at defined Cryba4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.