Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
β-2-Microglobulin Double Nickase Plasmid (h): sc-417704-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- β-2-Microglobulin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- β-2-Microglobulin Double Nickase Plasmid (h) and β-2-Microglobulin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting B2M. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: β-2-Microglobulin Antibody (G-10): sc-46697
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
β-2-Microglobulin Double Nickase Plasmid (h) | sc-417704-NIC | 20 µg | $410.00 | |||
β-2-Microglobulin Double Nickase Plasmid (h2) | sc-417704-NIC-2 | 20 µg | $410.00 |
B2M encodes β-2-microglobulin, a non-covalent component of MHC class I complexes that is required for proper folding, stability, and cell-surface presentation of HLA class I molecules. By supporting peptide presentation to CD8+ T cells, β-2-microglobulin influences antigen processing and presentation pathways and shapes immune surveillance and self–nonself recognition. Altered B2M expression or loss-of-function can disrupt MHC I trafficking and impair antigen presentation, a feature observed in multiple immune-evasion contexts. These properties make B2M a widely used marker and functional node for interrogating HLA-dependent signaling, interferon-responsive programs, and immune–tumor interactions in human cell models.
β-2-Microglobulin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the B2M locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within B2M. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt B2M function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of B2M-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.