Molecular structure of Puromycin dihydrochloride, CAS Number: 58-58-2
Puromycin dihydrochloride (CAS 58-58-2)
See product citations (394)
Alternate Names:
Puromycin dihydrochloride is also known as Stylomycin hydrochloride
Application:
Puromycin dihydrochloride is an antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene
CAS Number:
58-58-2
Purity:
≥98%
Molecular Weight:
544.43
Molecular Formula:
C22H29N7O5•2HCl
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Puromycin dihydrochloride, also known as Stylomycin hydrochloride, is an aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene, whose product, puromycin-N-acetyl-transferase, inactivates puromycin via acetylation. Puromycin dihydrochloride has been cell culture tested and is recommended for selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles. Puromycin dihydrochloride is an antibiotic substance produced by the soil actinomycete Streptomyces alboniger which induces apoptosis in cells by interfering with RNA function, leading to inhibition of protein synthesis. Puromycin dihydrochloride is thought to act as an acyl-tRNA analogue causing premature chain termination. Toxic to both eukaryotic and prokaryotic cells, Puromycin dihydrochloride has also been shown to arrest cells in G2/M phase.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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Puromycin dihydrochloride (CAS 58-58-2) References
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- Pathophysiology and treatment of focal segmental glomerulosclerosis: the role of animal models. | de Mik, SM., et al. 2013. BMC Nephrol. 14: 74. PMID: 23547922
- The hypusine-containing translation factor eIF5A. | Dever, TE., et al. 2014. Crit Rev Biochem Mol Biol. 49: 413-25. PMID: 25029904
- Genetic associations and functional characterization of M1 aminopeptidases and immune-mediated diseases. | Agrawal, N. and Brown, MA. 2014. Genes Immun. 15: 521-7. PMID: 25142031
- Therapeutic target for nephrotic syndrome: Identification of novel slit diaphragm associated molecules. | Fukusumi, Y., et al. 2014. World J Nephrol. 3: 77-84. PMID: 25332898
- Regulatory T cells and minimal change nephropathy: in the midst of a complex network. | Bertelli, R., et al. 2016. Clin Exp Immunol. 183: 166-74. PMID: 26147676
- Antibiotics and RNase P. | Drainas, D. 2016. Antibiotics (Basel). 5: PMID: 27164152
- Chloramphenicol Derivatives as Antibacterial and Anticancer Agents: Historic Problems and Current Solutions. | Dinos, GP., et al. 2016. Antibiotics (Basel). 5: PMID: 27271676
- Combined use of electron microscopy and intravital imaging captures morphological and functional features of podocyte detachment. | Burford, JL., et al. 2017. Pflugers Arch. 469: 965-974. PMID: 28664407
- Transient and stable vector transfection: Pitfalls, off-target effects, artifacts. | Stepanenko, AA. and Heng, HH. 2017. Mutat Res Rev Mutat Res. 773: 91-103. PMID: 28927539
- Spontaneous and glucocorticoid-induced apoptosis in human mature T lymphocytes. | Brunetti, M., et al. 1995. Blood. 86: 4199-205. PMID: 7492778
- Puromycin-sensitive aminopeptidase. Sequence analysis, expression, and functional characterization. | Constam, DB., et al. 1995. J Biol Chem. 270: 26931-9. PMID: 7592939
- Puromycin induces apoptosis of developing chick sympathetic neurons in a similar manner to NGF-deprivation. | Sugita, M., et al. 1995. Zoolog Sci. 12: 419-25. PMID: 8528014
- sc-Labeled 1,4,7,10-Tetraazacyclododecane-1,4,7,10-Tetraacetic Acid–Conjugated Puromycin | Arvind Chopra. 2004. Molecular Imaging And Contrast Agent Database.
Inhibitor of:
α3e Tubulin, α8 Tubulin, κ-Actin, 0610009B22Rik, 2610027L16Rik, 82-FIP, AARSD1, ADAT2, AlaRS, ANKRD18A, ARD1B, arfaptin 1, ARMC3, ASAH2C, ATP9BL, ATPBD1B, ATPBD3, ATXN3L, BASE, BFZB, BLOS1, BTF3L4, BTF3L4P, BTN1A1, C10orf132, C10orf50, C10orf51, C14orf108, C14orf149, C15orf62, C1orf109, C1orf114, C1orf127, C1orf77, C1orf85, C21orf70, C3orf26, C3orf49, C3orf62, C8orf13, C8orf33, C8orf44, C8orf48, C8orf58, C8orf74, C8orf76, Caprin1, CBWD, CCDC66, CD39L3, CDRT15L, CENP-S, CES4, CLCA4_Clac4, CLTCL1, COL12A, Coronin 7, CR1L, cryptdin 20, cryptdin 6, CSHL1, CTBS, CTF8, CTGLF5, CXorf20, CXorf39, Cyclon, CYP4F12, CYP4F8, CYP4V3, CysRS, D930020E02Rik, DDX26B, DDX54, DGCR6L, Dim1, DPH5, Ear12, EF-1 ε1, EF-1δP1, EG432502, EG435337, EG621697, Elastase-2B, EMG1, Emp, ERAP1, ERC2, eRF3b, Ermin, ERVK6, EVPLL, FABP5L1, FABP5L2, FABP5L7, FAM11B, FAM137B, FAM139A, FAM86B1, FAM90A13, FAM90A14, FAM90A16, FAM90A17, FAM90A18, FAM90A19, FAM90A20, FAM90A3, FAM90A5, FAM90A8, FAM90A9, FAM92A1, FBXO24, FDXACB1, FLJ44635, fushi tarazu, G3BP, GAGE2B, GAL3ST4, GBP7, Get4, GIGYF2, GlnRS, GluRS, Glutamine-rich 1, Gm14431, GMEB-1, Grcc10, GSTA5, hCG_15200, hCG_1781062, hCG_2015138, hCG_2024596, HSPA6, HSPC105, HSPC152, IF2, IleRS, INS-IGF2, JTV1, KIAA0090, KIAA0329, Kizuna_Plk1s1, KLHDC3, KRTAP1-4, KRTAP2-1, KRTAP9-L1, KY peptidase, LARS2, LeuRS, LOC198437, LOC399839, LOC645359, LOC645967, LOC646736, LOC647166, LOC729978, LOC729991, LOC730098, LSG1, LSm14B, MAGOHB, MARS2, Maskin, megalin, MESDC1, Mex3c, MFSD11, MRC1L1, MRCL3, MRP-L1, MRPL28, MTFmt, Myosin VI, Myosin-15, Nibrin, NIP7, Olfr417, Olfr714, Olfr790, Olfr807, Olfr810, Olfr981, OR8D4, oskar, OTTMUSG00000010657, OTTMUSG00000016414, OTTMUSG00000016437, PABPC1L, PABPC4L, Paip2b, Pcdbh6, Pcdhb18, pki γ, PMFBP1, POTE15, POTE8, PRH1, RALYL, RBM27, RBM32A, RBMY1J, RBP, RCBTB1, RCOR3, RGPD5, RGPD6, RGPD8, Ribosomal Protein L10L, Ribosomal Protein L14, Ribosomal Protein L23, Ribosomal Protein L29, Ribosomal Protein L32, Ribosomal Protein L36a, Ribosomal Protein L36aL, Ribosomal Protein L38, Ribosomal Protein L5, Ribosomal Protein S21, Ribosomal Protein S25, Ribosomal Protein S27L, Ribosomal Protein S3, Ribosomal Protein S8, Ribosomal ProteinL18, Rim4, RNase 2, RNF170, ROPN1B, RPL10L, RPL26L1, RPUSD3, Rrp6, S19BP, SELB, SKIV2L2, Snrp116, SPAG11A, STAU2, SURF-2, SUSD5, Synaptogyrin-2, Synaptotagmin VI, SYS1, TBC1D22A, TCEB1P3, TCP-1 ε, TCP-1 ζ2, Teneurin-1, TEX28, TFIIH p44L, THUMPD1, tinman, TINP1, TIS11B, TMEM162, TMEM183A, TMEM2, TMEM65, TMEM91, TSEN2, TSEN34, TSR1, TTC21A, TTC22, TTC37, Ub-RPS27A, UBE2W, Ubie, Ubie3, UBL4B, UGT2B34, UGT2B35, UTP14C, v-SNARE Vti1p, V1RA7, V1RA8, V1RB3, Vgl-4, VSTM2L, WDR61, YLAT1, Ym2, ZBED4, ZCCHC14, ZFHX4, ZFP192, ZFP213, ZFP26, and ZNF213.Activator of:
AsnRS, BMS1, CIRH1A, DIS3L2, EDEM, EG432502, EIF3S6IP, eIF4AI, eIF5B, eRF3a, ETEA, FAM98A, FASP1, GNL2, granzyme N, hDcp1a, LTV1, LysRS, MRP-L38, Nop2p, NVL, PABPC3, PABPC4L, PABPNL1, Paip2, pelota, QM, RBM47, Rent2, Ribosomal Protein L10L, Ribosomal Protein L11, Ribosomal Protein L12P8, Ribosomal Protein L13, Ribosomal Protein L17, Ribosomal Protein L22, Ribosomal Protein L27a, Ribosomal Protein L38, Ribosomal Protein L39, Ribosomal Protein L3L, Ribosomal Protein L6, Ribosomal Protein LP0, Ribosomal Protein S19, Ribosomal Protein S23, Ribosomal Protein S24, Ribosomal Protein S26, RPL10L, RPL32, RPUSD1, SCCA2, SRP54b, SRP68, TDE1, TRUB2, U2 snRNP A, and WDR3.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Puromycin dihydrochloride, 25 mg | sc-108071 | 25 mg | $40.00 | |||
Puromycin dihydrochloride, 50 mg | sc-108071A | 50 mg | $65.00 | |||
Puromycin dihydrochloride, 250 mg | sc-108071B | 250 mg | $210.00 | |||
Puromycin dihydrochloride, 1 g | sc-108071C | 1 g | $816.00 |