Zscan4c Double Nickase Plasmid (m): sc-434121-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZSCAN4C Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZSCAN4C Double Nickase Plasmid (m) and ZSCAN4C Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Zscan4c. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Zscan4c Double Nickase Plasmid (m) | sc-434121-NIC | 20 µg | $410.00 |
Zscan4c (zinc finger and SCAN domain containing 4C) is a mouse transcription factor predominantly associated with the two-cell–like state in early embryogenesis and subsets of pluripotent stem cells. It is implicated in genome stability programs, including telomere length regulation, DNA repair, and chromatin remodeling, and has been linked to activation of cleavage-stage transcriptional networks. Zscan4 family activity is commonly studied in the context of epigenetic reprogramming, totipotency-associated gene expression, and maintenance of genomic integrity under replicative stress. Dysregulation of these processes is relevant to developmental abnormalities and genome instability phenotypes that can inform models of oncogenic transformation and stem cell dysfunction.
ZSCAN4C Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Zscan4c locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Zscan4c. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Zscan4c function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Zscan4c-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.