ZNF384 Double Nickase Plasmid (h): sc-414941-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZNF384 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZNF384 Double Nickase Plasmid (h) and ZNF384 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZNF384. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZNF384 Antibody (3545C5a): sc-517543
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF384 Double Nickase Plasmid (h) | sc-414941-NIC | 20 µg | $410.00 |
ZNF384 encodes a zinc finger transcription factor that binds DNA and modulates gene expression programs involved in cell growth, differentiation, and lineage specification. It participates in transcriptional regulation networks that intersect with chromatin organization and RNA processing, influencing context-dependent promoter and enhancer activity. In hematopoietic cells, dysregulation of ZNF384 has been linked to recurrent gene fusions and altered transcriptional circuitry that can rewire developmental pathways. These features make ZNF384 a useful target for studying transcription factor–driven gene regulatory networks and their effects on cellular identity.
ZNF384 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZNF384 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZNF384. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZNF384 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZNF384-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.