ZnAF-2 tetrahydrochloride MF: C34H28N4O5•4HCl
MW: 718.45
A high-affinity Zn2+-specific fluorescent probe.

ZnAF-2 tetrahydrochloride

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Alternate Names: 6-{2-[Bis(2-pyridylmethyl)amino]ethylamino}fluorescein Tetrahydrochloride
Application: ZnAF-2 tetrahydrochloride is a high-affinity Zn2+-specific fluorescent probe
Molecular Weight: 718.45
Molecular Formula: C34H28N4O54HCl
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

ZnAF-2 is a membrane-impermeable ethylenediaminofluoresceinyl compound that acts as a high-affinity Zn2+-specific fluorescent probe (Kd = 2.7 nM). It has a low basal fluorescence which increases in intensity ~51-fold upon stoichiometric (1:1) binding to Zn2+. ZnAF-2 tetrahydrochloride exhibits little affinity towards Ca2+, Na+, Mg2+, or K+. ZnAF-2 has been used successfully in monitoring spatio-temporal dynamics of extracellular Zn2+ release in rat hippocampal samples. A cell-permeable, diacetylated form of ZnAF-2 (sc-216074) is also available for intracellular use.


References

1. Hirano, Tomoya., et al., 2002. Improvement and biological applications of fluorescent probes for zinc, ZnAFs. Journal of the American Chemical Society. 124(23): 6555-62. PMID: 12047174
2. Ueno, Sayaka., et al., 2002. Mossy fiber Zn2+ spillover modulates heterosynaptic N-methyl-D-aspartate receptor activity in hippocampal CA3 circuits. The Journal of cell biology. 158(2): 215-20. PMID: 12119362

Formulation :
~5mM in 0.28mL in DMSO
Physical State :
Liquid
Solubility :
Soluble in DMSO (5 mM).
Storage :
Store at -20° C
Refractive Index :
n20D 1.74 (Predicted)
Fluoresence :
λex ~492 nm, λem ~514 nm in DMSO
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
16218345
SMILES :
C1=CC=NC(=C1)CN(CCNC2=CC(=C(C=C2)C(=O)O)C3=C4C=CC(=O)C=C4OC5=C3C=CC(=C5)O)CC6=CC=CC=N6.Cl.Cl.Cl.Cl

Download SDS (MSDS)

Certificate of Analysis

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