ZnAF-2 is a membrane-impermeable ethylenediaminofluoresceinyl compound that acts as a high-affinity Zn2+-specific fluorescent probe (Kd = 2.7 nM). It has a low basal fluorescence which increases in intensity ~51-fold upon stoichiometric (1:1) binding to Zn2+. ZnAF-2 tetrahydrochloride exhibits little affinity towards Ca2+, Na+, Mg2+, or K+. ZnAF-2 has been used successfully in monitoring spatio-temporal dynamics of extracellular Zn2+ release in rat hippocampal samples. A cell-permeable, diacetylated form of ZnAF-2 (sc-216074) is also available for intracellular use.
1. Hirano, Tomoya., et al., 2002. Improvement and biological applications of fluorescent probes for zinc, ZnAFs. Journal of the American Chemical Society. 124(23): 6555-62. PMID: 12047174
2. Ueno, Sayaka., et al., 2002. Mossy fiber Zn2+ spillover modulates heterosynaptic N-methyl-D-aspartate receptor activity in hippocampal CA3 circuits. The Journal of cell biology. 158(2): 215-20. PMID: 12119362
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