ZKSCAN1 Double Nickase Plasmid (m): sc-428933-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZKSCAN1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZKSCAN1 Double Nickase Plasmid (m) and ZKSCAN1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Zkscan1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZKSCAN1 Double Nickase Plasmid (m) | sc-428933-NIC | 20 µg | $410.00 |
Zkscan1 encodes ZKSCAN1, a KRAB and SCAN domain–containing C2H2 zinc-finger transcription factor implicated in sequence-specific gene regulation and chromatin-associated transcriptional control. Through its KRAB-mediated recruitment of corepressor complexes, ZKSCAN1 is linked to epigenetic repression programs that influence cell-state maintenance, proliferation, and stress-responsive transcriptional networks. Altered ZKSCAN1 expression or activity has been studied in contexts of dysregulated transcription and oncogenic signaling, where perturbations can impact pathways controlling cell cycle progression and differentiation. In mouse models, Zkscan1 provides a tractable locus for dissecting how KRAB-ZFP regulators shape gene expression programs in development and disease-relevant cellular phenotypes.
ZKSCAN1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Zkscan1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Zkscan1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Zkscan1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Zkscan1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.