Zic3 Antibody (SQ-15): sc-101201

Zic3 Antibody (SQ-15) is a mouse monoclonal IgG2a kappa light chain antibody that detects Zic3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Zic3 antibody (SQ-15) is available as non-conjugated format. Zic3, known as zinc finger protein of the cerebellum 3, plays a crucial role as a C2H2 zinc finger transcription factor, essential for establishing proper left-right axis and midline neural patterning during early development of vertebrate embryos. Zic3 influences development of various organ systems, and mutations in Zic3 gene can lead to severe developmental disorders, including X-linked visceral heterotaxy, characterized by congenital heart disease and defects in left-right axis of organs. Mutations affecting zinc finger DNA binding domain and N-terminal domain of Zic3 result in loss of transactivation capability for reporter genes, while specific mutations within amino acid range 253-323 lead to aberrant cytoplasmic localization instead of normal nuclear localization, further underscoring Zic3′s significance in developmental biology and potential implications in congenital malformations.

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Zic3 Antibody (SQ-15) References:

1. Transcription factors Zic1 and Zic2 bind and transactivate the apolipoprotein E gene promoter.  |  Salero, E., et al. 2001. J Biol Chem. 276: 1881-8. PMID: 11038359
2. Behavioral abnormalities of Zic1 and Zic2 mutant mice: implications as models for human neurological disorders.  |  Ogura, H., et al. 2001. Behav Genet. 31: 317-24. PMID: 11699604
3. Zic1 represses Math1 expression via interactions with the Math1 enhancer and modulation of Math1 autoregulation.  |  Ebert, PJ., et al. 2003. Development. 130: 1949-59. PMID: 12642498
4. The role of ZIC3 in vertebrate development.  |  Herman, GE. and El-Hodiri, HM. 2002. Cytogenet Genome Res. 99: 229-35. PMID: 12900569
5. Disruption of gradient expression of Zic3 resulted in abnormal intra-retinal axon projection.  |  Zhang, J., et al. 2004. Development. 131: 1553-62. PMID: 14985256
6. Heterozygous deletion of the linked genes ZIC1 and ZIC4 is involved in Dandy-Walker malformation.  |  Grinberg, I., et al. 2004. Nat Genet. 36: 1053-5. PMID: 15338008
7. Zic3 is critical for early embryonic patterning during gastrulation.  |  Ware, SM., et al. 2006. Dev Dyn. 235: 776-85. PMID: 16397896
8. Zic3 is required in the migrating primitive streak for node morphogenesis and left-right patterning.  |  Sutherland, MJ., et al. 2013. Hum Mol Genet. 22: 1913-23. PMID: 23303524
9. ZIC3 Controls the Transition from Naive to Primed Pluripotency.  |  Yang, SH., et al. 2019. Cell Rep. 27: 3215-3227.e6. PMID: 31189106
10. Loss of Zic3 impairs planar cell polarity leading to abnormal left-right signaling, heart defects and neural tube defects.  |  Bellchambers, HM. and Ware, SM. 2021. Hum Mol Genet. 30: 2402-2415. PMID: 34274973
11. ZIC2 and ZIC3 promote SWI/SNF recruitment to safeguard progression towards human primed pluripotency.  |  Hossain, I., et al. 2024. Nat Commun. 15: 8539. PMID: 39358345
12. The expression of the mouse Zic1, Zic2, and Zic3 gene suggests an essential role for Zic genes in body pattern formation.  |  Nagai, T., et al. 1997. Dev Biol. 182: 299-313. PMID: 9070329

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Zic3 Antibody (SQ-15) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Zic3 Antibody (SQ-15) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Zic3 Antibody (SQ-15) Specifications

Alternate Product Name: Zic3

Product Clone Name: SQ-15

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 182-274

Epitope Target: Zic3

Epitope Species: human

Product Reactivity/Detection: Zic3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Zic3 Antibody (SQ-15): N/A