ZBTB5 CRISPR/Cas9 KO Plasmid (h): sc-411389
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZBTB5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZBTB5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZBTB5 CRISPR/Cas9 KO Plasmid (h) | sc-411389 | 20 µg | $397.00 |
Overview
ZBTB5 (zinc finger and BTB domain containing 5) encodes a BTB/POZ domain transcription factor that binds DNA via C2H2 zinc fingers and is thought to act as a sequence-specific regulator of gene expression through recruitment of corepressor and chromatin-modifying complexes. By influencing transcriptional programs linked to cell-cycle progression, differentiation, and epigenetic state, ZBTB5 can shape lineage-specific gene networks and cellular homeostasis. Dysregulated expression or altered activity of BTB–zinc finger proteins is frequently associated with oncogenic transcriptional reprogramming and genome-wide changes in chromatin accessibility, making ZBTB5 a useful target for mechanistic studies in cancer and proliferative biology. In human cell models, perturbing ZBTB5 helps interrogate how transcriptional repression/activation interfaces with chromatin remodeling, DNA replication timing, and stress-response pathways.
ZBTB5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZBTB5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZBTB5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZBTB5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZBTB5 protein expression.
This CRISPR knockout system enables efficient generation of ZBTB5-deficient cell models for investigation of ZBTB5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZBTB5 exon(s) critical for ZBTB5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZBTB5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZBTB5 CRISPR/Cas9 KO Plasmid (h) and ZBTB5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZBTB5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZBTB5 HDR Plasmid (h) and ZBTB5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZBTB5 homology arms to support homology-directed repair at defined ZBTB5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.