Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ZBTB11 Double Nickase Plasmid (h): sc-411959-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZBTB11 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZBTB11 Double Nickase Plasmid (h) and ZBTB11 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZBTB11. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZBTB11 Double Nickase Plasmid (h) | sc-411959-NIC | 20 µg | $410.00 |
ZBTB11 encodes a BTB/POZ domain–containing zinc finger transcription factor that functions in chromatin-associated gene regulation and maintenance of appropriate transcriptional programs. Through sequence-specific DNA binding and recruitment of corepressor or chromatin-modifying complexes, ZBTB11 influences cellular processes such as proliferation, differentiation, and stress-responsive transcription. Perturbation of BTB-zinc finger transcriptional regulators can remodel epigenetic states and impact genome stability–linked pathways, making ZBTB11 a useful node for studying transcriptional control in human cells. Dysregulated transcription factor networks involving ZBTB family proteins have been implicated in oncogenic and developmental phenotypes, supporting research into disease-relevant regulatory circuitry without implying clinical utility.
ZBTB11 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZBTB11 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZBTB11. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZBTB11 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZBTB11-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.