Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ZAG Double Nickase Plasmid (h): sc-401706-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZAG Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZAG Double Nickase Plasmid (h) and ZAG Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AZGP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZAG Antibody (1D4): sc-13585
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZAG Double Nickase Plasmid (h) | sc-401706-NIC | 20 µg | $410.00 | |||
ZAG Double Nickase Plasmid (h2) | sc-401706-NIC-2 | 20 µg | $410.00 |
AZGP1 encodes zinc-α2-glycoprotein (ZAG), a secreted, MHC class I–like glycoprotein implicated in lipid mobilization and systemic energy homeostasis. ZAG is produced by epithelial and adipose-associated tissues and has been linked to regulation of adipocyte metabolism, extracellular signaling, and inflammatory crosstalk within the tissue microenvironment. Altered AZGP1 expression has been reported across multiple cancer types and metabolic conditions, where it correlates with changes in differentiation state, nutrient utilization, and catabolic programs. These associations make AZGP1/ZAG a useful marker and mechanistic node for studying metabolic remodeling, epithelial biology, and tumor–stroma interactions.
ZAG Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AZGP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AZGP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AZGP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AZGP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.