Date published: 2025-12-18

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Z-VAD-FMK (CAS 187389-52-2)

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Application:
Z-VAD-FMK is Irreversible, cell-permeable, broad-spectrum caspase Inhibitor
CAS Number:
187389-52-2
Purity:
≥95%
Molecular Weight:
467.49
Molecular Formula:
C22H30FN3O7
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Z-VAD-FMK is an irreversible, cell-permeable, broad-spectrum caspase inhibitor. Z-VAD-FMK limited Fas-induced mouse liver damage and apoptosis to


Z-VAD-FMK (CAS 187389-52-2) References

  1. Monocytic cell necrosis is mediated by potassium depletion and caspase-like proteases.  |  Warny, M. and Kelly, CP. 1999. Am J Physiol. 276: C717-24. PMID: 10070000
  2. TNF-induced enterocyte apoptosis and detachment in mice: induction of caspases and prevention by a caspase inhibitor, ZVAD-fmk.  |  Piguet, PF., et al. 1999. Lab Invest. 79: 495-500. PMID: 10212002
  3. Correlation of tumor necrosis factor alpha (TNF alpha) with high Caspase 3-like activity in myelodysplastic syndromes.  |  Mundle, SD., et al. 1999. Cancer Lett. 140: 201-7. PMID: 10403560
  4. A specific inhibitor of apoptosis decreases tissue injury after intestinal ischemia-reperfusion in mice.  |  Farber, A., et al. 1999. J Vasc Surg. 30: 752-60. PMID: 10514215
  5. Protective effect of a caspase inhibitor in models for cerebral ischemia in vitro and in vivo.  |  Wiessner, C., et al. 2000. Cell Mol Biol (Noisy-le-grand). 46: 53-62. PMID: 10726971
  6. Apoptosis in Alzheimer's disease--an update.  |  Shimohama, S. 2000. Apoptosis. 5: 9-16. PMID: 11227497
  7. Nitric oxide-induced mitochondrial fission is regulated by dynamin-related GTPases in neurons.  |  Barsoum, MJ., et al. 2006. EMBO J. 25: 3900-11. PMID: 16874299
  8. GDC-0152-induced autophagy promotes apoptosis in HL-60 cells.  |  Peiqi, L., et al. 2018. Mol Cell Biochem. 445: 135-143. PMID: 29299747
  9. Involvement of multiple proteases during Fas-mediated apoptosis in T lymphocytes.  |  Chow, SC., et al. 1995. FEBS Lett. 364: 134-8. PMID: 7538469
  10. An ICE-like protease is a common mediator of apoptosis induced by diverse stimuli in human monocytic THP.1 cells.  |  Zhu, H., et al. 1995. FEBS Lett. 374: 303-8. PMID: 7589559
  11. Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32.  |  Slee, EA., et al. 1996. Biochem J. 315 (Pt 1): 21-4. PMID: 8670109
  12. Activation of PKCalpha downstream from caspases during apoptosis induced by 7-hydroxystaurosporine or the topoisomerase inhibitors, camptothecin and etoposide, in human myeloid leukemia HL60 cells.  |  Shao, RG., et al. 1997. J Biol Chem. 272: 31321-5. PMID: 9395460
  13. Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver.  |  Chandler, JM., et al. 1998. J Biol Chem. 273: 10815-8. PMID: 9556551

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Z-VAD-FMK, 500 µg

sc-3067
500 µg
$74.00

Can we use the inhibitor in a whole zebrafish? Which conditions? Thank you.

Asked by: RaquelL
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2021-10-11

Is zVAD-FMK stable in full media at 37°C for 72h? I want to perform a viablility assay and block apoptosis to see if it can rescue my treatment. Can I leave zVAD in the media for 72h or do I have to change it every 24h or so?

Asked by: Leo1908
Thank you for your question. Please contact our technical service directly so we can address your particular experiment.
Answered by: Technical Support Europe
Date published: 2019-03-08

Is it possible to use this product to inhibit purified caspases?

Asked by: Adam1215
Thank you for your question. Z-VAD-FMK (CAS 187389-52-2) is an irreversible, cell-permeable, broad-spectrum caspase inhibitor. For information about how other researchers have used this chemical in their research, please refer to the list of product references on our website (listed below the Description, below the ordering information).
Answered by: Technical Support
Date published: 2017-01-03

What is the solubility of this product?

Asked by: hawkeye11
Thank you for your question. This chemical is soluble in DMSO at 20mM concentration. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-22
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Rated 5 out of 5 by from Good qualityGood dose dependent inhibition of receptor mediated cell death in human B cell lines
Date published: 2018-07-23
Rated 5 out of 5 by from Excellent inhibitorThe product is useful, with good result in our research. Act on target molecules effectively.
Date published: 2016-12-06
Rated 5 out of 5 by from Great production!The inhibitor totally fit our experiment and we'd like to buy it again.
Date published: 2016-11-02
Rated 5 out of 5 by from The inhibitor works wellThe inhibitor works well. It can inhibit the target molecule effectively.I will continue purchase your other inhibitor for further use.
Date published: 2015-10-15
Rated 5 out of 5 by from Inhibitor worked well for our cell assaysInhibitor worked well for our cell assays.
Date published: 2015-09-28
Rated 5 out of 5 by from Produced optimal results in our cell culturesProduced optimal results in our cell cultures.
Date published: 2015-09-23
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Z-VAD-FMK is rated 5.0 out of 5 by 6.
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