Y79 + PMA nuclear extract: sc-2127
- supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer
- provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT
- human nuclear extract; retinoblastoma cells induced with phorbol
- suitable for use in Gel Shift and Western Blotting assays
- Extracts should be stored at -70°C and repeated freezing and thawing should be avoided.
- prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475
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SEE ALSO...
Y79 + PMA nuclear extract combines the Y79 retinoblastoma cell line with Phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C (PKC), to study signal transduction mechanisms and gene expression modulation in a research setting. This specialized nuclear extract is utilized primarily to analyze the effects of PKC activation on nuclear factors and pathways involved in cell cycle regulation and gene transcription specific to retinal cells. Researchers employ this extract in various experimental setups including kinase assays, reporter gene assays, and chromatin immunoprecipitation, to observe the direct influence of PKC activation on chromatin structure and function, transcription factor activity, and the expression of genes linked to cellular proliferation and differentiation. The combination of Y79 cells with PMA provides a unique tool for dissecting the role of PKC in cellular mechanisms underlying oncogenic processes, especially in the context of retinal biology. This extract is particularly valuable for its application in research aimed at understanding fundamental biological processes, ensuring that all findings are strictly attributable to scientific inquiry. Verification of the Y79 cell line origin and handling is crucial for maintaining the integrity and reproducibility of research outcomes.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Y79 + PMA nuclear extract | sc-2127 | 250 µg/0.05 ml | $160.00 |