Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
XPC Double Nickase Plasmid (h): sc-401499-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- XPC Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- XPC Double Nickase Plasmid (h) and XPC Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting XPC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: XPC Antibody (D-10): sc-74410
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XPC Double Nickase Plasmid (h) | sc-401499-NIC | 20 µg | $410.00 | |||
XPC Double Nickase Plasmid (h2) | sc-401499-NIC-2 | 20 µg | $410.00 |
XPC encodes the xeroderma pigmentosum group C protein, a central damage sensor in global genome nucleotide excision repair (GG-NER) that detects helix-distorting DNA lesions such as UV-induced cyclobutane pyrimidine dimers and bulky chemical adducts. Upon lesion recognition, XPC coordinates recruitment of TFIIH and downstream NER factors to initiate DNA unwinding, excision, and resynthesis, thereby supporting genome integrity and replication fork stability. XPC function interfaces with DNA damage signaling and cell-cycle checkpoint control, influencing cellular responses to genotoxic stress. Genetic defects in XPC underlie xeroderma pigmentosum complementation group C and are associated with elevated mutational burden and heightened sensitivity to UV and environmental mutagens.
XPC Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the XPC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within XPC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt XPC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of XPC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.