XEDAR Antibody (G-5): sc-377423
- XEDAR Antibody (G-5) is a mouse monoclonal IgG1 κ XEDAR antibody provided at 200 µg/ml
- raised against amino acids 220-297 mapping at the C-terminus of XEDAR of human origin
- XEDAR Antibody (G-5) is recommended for detection of XEDAR of human origin by WB, IP, IF and ELISA
- Anti-XEDAR Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for XEDAR Antibody (G-5) for WB applications. This reagent is now offered in a bundle with XEDAR Antibody (G-5) (see ordering information below).
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XEDAR Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects XEDAR protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-XEDAR antibody (G-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The ectodermal dysplasia receptor (EDAR) plays a crucial role in ectodermal differentiation and is part of the tumor necrosis factor receptor (TNFR) superfamily, which consists of type I transmembrane glycoproteins involved in essential cellular functions such as proliferation, differentiation, and programmed cell death. XEDAR contains cysteine-rich repeats in its extracellular ligand-binding domain and an intracellular death domain, which are critical for mediating signaling pathways. These pathways can lead to either cell survival through the NFkB pathway or cell death via the caspase and Jun kinase pathways. Mutations in the gene encoding EDAR can result in ectodermal dysplasia syndromes, which are characterized by developmental issues in hair, teeth, and sweat glands. The interaction between Ectodysplasin A (EDA), a type II membrane protein encoded by the Tabby gene, and EDAR is vital for normal ectodermal development, with EDA-A1 serving as the primary ligand for EDAR, while EDA-A2 specifically binds to XEDAR. Both EDAR and XEDAR share homology with TROY, highlighting their evolutionary significance and functional interconnections within the TNFR superfamily.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
XEDAR Antibody (G-5) References:
- TROY, a newly identified member of the tumor necrosis factor receptor superfamily, exhibits a homology with Edar and is expressed in embryonic skin and hair follicles. | Kojima, T., et al. 2000. J Biol Chem. 275: 20742-7. PMID: 10764796
- Edar/Eda interactions regulate enamel knot formation in tooth morphogenesis. | Tucker, AS., et al. 2000. Development. 127: 4691-700. PMID: 11023871
- The ectodermal dysplasia receptor activates the nuclear factor-kappaB, JNK, and cell death pathways and binds to ectodysplasin A. | Kumar, A., et al. 2001. J Biol Chem. 276: 2668-77. PMID: 11035039
- Two-amino acid molecular switch in an epithelial morphogen that regulates binding to two distinct receptors. | Yan, M., et al. 2000. Science. 290: 523-7. PMID: 11039935
- Ectodysplasin is released by proteolytic shedding and binds to the EDAR protein. | Elomaa, O., et al. 2001. Hum Mol Genet. 10: 953-62. PMID: 11309369
- Structural and biological features of the TNF receptor and TNF ligand superfamilies: interactive signals in the pathobiology of Hodgkin's disease. | Gruss, HJ., et al. 1996. Ann Oncol. 7 Suppl 4: 19-26. PMID: 8836404
- Molecular, structural, and biological characteristics of the tumor necrosis factor ligand superfamily. | Gruss, HJ. 1996. Int J Clin Lab Res. 26: 143-59. PMID: 8905447
- Modulation of life and death by the TNF receptor superfamily. | Baker, SJ. and Reddy, EP. 1998. Oncogene. 17: 3261-70. PMID: 9916988
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XEDAR Antibody (G-5) | sc-377423 | 200 µg/ml | $322.00 | |||
XEDAR Antibody (G-5): m-IgG1 BP-HRP Bundle | sc-531732 | 200 µg Ab; 20 µg BP | $361.00 | |||
XEDAR Antibody (G-5) AC | sc-377423 AC | 500 µg/ml, 25% agarose | $424.00 | |||
XEDAR Antibody (G-5) HRP | sc-377423 HRP | 200 µg/ml | $322.00 | |||
XEDAR Antibody (G-5) FITC | sc-377423 FITC | 200 µg/ml | $336.00 | |||
XEDAR Antibody (G-5) PE | sc-377423 PE | 200 µg/ml | $349.00 | |||
XEDAR Antibody (G-5) Alexa Fluor® 488 | sc-377423 AF488 | 200 µg/ml | $364.00 | |||
XEDAR Antibody (G-5) Alexa Fluor® 546 | sc-377423 AF546 | 200 µg/ml | $364.00 | |||
XEDAR Antibody (G-5) Alexa Fluor® 594 | sc-377423 AF594 | 200 µg/ml | $364.00 | |||
XEDAR Antibody (G-5) Alexa Fluor® 647 | sc-377423 AF647 | 200 µg/ml | $364.00 | |||
XEDAR Antibody (G-5) Alexa Fluor® 680 | sc-377423 AF680 | 200 µg/ml | $364.00 | |||
XEDAR Antibody (G-5) Alexa Fluor® 790 | sc-377423 AF790 | 200 µg/ml | $364.00 |