WT1 Antibody (F-6): sc-7385

PKC ζ Antibody (H-1) is a mouse monoclonal IgG2a antibody that targets an epitope mapping between amino acids 573-592 at the C-terminus of rat-origin Protein Kinase C zeta (PKC ζ), also known as PRKCZ or nPKC-ζ. PKC ζ monoclonal antibody (H-1) reacts with mouse, rat, and human species and is suitable for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PKC ζ is an atypical member of the protein kinase C family, playing a crucial role in various cellular processes such as cell growth, differentiation, survival, gene expression, and membrane function. Unlike conventional PKCs, PKC ζ activation is independent of calcium and diacylglycerol (DAG), allowing unique function within signaling pathways. PKC ζ is involved in the regulation of NF-κB activation, insulin signaling, cell polarity, and the PI3K/Akt pathway, making PKC ζ significant in understanding diseases like diabetes, cancer, and immune disorders. PKC ζ involvement in cell survival and proliferation underscores importance as a potential therapeutic target. Furthermore, distinct activation mechanisms make PKC ζ an intriguing subject in signal transduction research, potentially revealing new insights into cellular communication pathways. PKC ζ monoclonal antibody (H-1) enables researchers to effectively study expression and function in various biological contexts, aiding in the development of novel treatments.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

WT1 Antibody (F-6) References:

1. Zinc finger point mutations within the WT1 gene in Wilms tumor patients.  |  Little, MH., et al. 1992. Proc Natl Acad Sci U S A. 89: 4791-5. PMID: 1317572
2. Repression of the insulin-like growth factor II gene by the Wilms tumor suppressor WT1.  |  Drummond, IA., et al. 1992. Science. 257: 674-8. PMID: 1323141
3. The Wilms' tumor gene product, WT1, represses transcription of the platelet-derived growth factor A-chain gene.  |  Wang, ZY., et al. 1992. J Biol Chem. 267: 21999-2002. PMID: 1429549
4. Characterization of the zinc finger protein encoded by the WT1 Wilms' tumor locus.  |  Morris, JF., et al. 1991. Oncogene. 6: 2339-48. PMID: 1662794
5. A tumor suppressor and oncogene: the WT1 story.  |  Yang, L., et al. 2007. Leukemia. 21: 868-76. PMID: 17361230
6. Isolation and characterization of a zinc finger polypeptide gene at the human chromosome 11 Wilms' tumor locus.  |  Call, KM., et al. 1990. Cell. 60: 509-20. PMID: 2154335
7. Homozygous deletion in Wilms tumours of a zinc-finger gene identified by chromosome jumping.  |  Gessler, M., et al. 1990. Nature. 343: 774-8. PMID: 2154702
8. Introduction of a normal human chromosome 11 into a Wilms' tumor cell line controls its tumorigenic expression.  |  Weissman, BE., et al. 1987. Science. 236: 175-80. PMID: 3031816
9. Genetics of Wilms' tumor.  |  Matsunaga, E. 1981. Hum Genet. 57: 231-46. PMID: 6265341

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-WT1 Antibody (F-6) has 154 citations in a variety of scientific publications.

Primary antibodies like Anti-WT1 Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

WT1 Antibody (F-6) Specifications

Alternate Product Name: WT1

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 172-228

Epitope Target: WT1

Epitope Species: human

Product Reactivity/Detection: WT1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 154 citations in various scientific publications

Available Conjugates/Forms for WT1 Antibody (F-6): AC: Agarose, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, X: TransCruz Reagent

The preferred WT1 antibody is WT1 Antibody (H-1): sc-393498.