Molecular structure of WST-8, CAS Number: 193149-74-5
WST-8 (CAS 193149-74-5)
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Alternate Names:
2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium Sodium Salt; 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazolium, inner salt, monosodium salt
Application:
WST-8 is a tetrazolium reagent that serves as a chromogenic indicator for NADH
CAS Number:
193149-74-5
Purity:
≥95%
Molecular Weight:
600.47
Molecular Formula:
C20H13N6NaO11S2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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Description
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SDS & Certificate of Analysis
WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nM. It is used as a cell viability indicator in cell proliferation assays.
WST-8 (CAS 193149-74-5) References
- Lymphocyte proliferation response during Eimeria tenella infection assessed by a new, reliable, nonradioactive colorimetric assay. | Miyamoto, T., et al. 2002. Avian Dis. 46: 10-6. PMID: 11922320
- Reactivity of blood samples spotted onto filter papers in the WST-8 method for screening of G6PD deficiency. | Arai, M., et al. 2006. Acta Med Okayama. 60: 127-34. PMID: 16680190
- Comparative evaluation of 2,3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium, monosodium salt (WST-8) rapid colorimetric assays for antimicrobial susceptibility testing of staphylococci and ESBL-producing clinical isolates. | Brady, AJ., et al. 2007. J Microbiol Methods. 71: 305-11. PMID: 17963904
- A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability. | Ishiyama, M., et al. 1997. Talanta. 44: 1299-305. PMID: 18966866
- Comparison of the WST-8 colorimetric method and the CLSI broth microdilution method for susceptibility testing against drug-resistant bacteria. | Tsukatani, T., et al. 2012. J Microbiol Methods. 90: 160-6. PMID: 22642794
- Spectrophotometric Assay for Superoxide Dismutase Based on the Reduction of Highly Water-soluble Tetrazolium Salts by Xanthine-Xanthine Oxidase. | Ukeda, H., et al. 1999. Biosci Biotechnol Biochem. 63: 485-8. PMID: 27393255
- High-content image analysis (HCIA) assay has the highest correlation with direct counting cell suspension compared to the ATP, WST-8 and Alamar blue assays for measurement of cytotoxicity. | Tahara, H., et al. 2017. J Pharmacol Toxicol Methods. 88: 92-99. PMID: 28807796
- Cytotoxicity of Shear Exfoliated Pnictogen (As, Sb, Bi) Nanosheets. | Chia, HL., et al. 2019. Chemistry. 25: 2242-2249. PMID: 30637810
- Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays. | Chamchoy, K., et al. 2019. BMC Biochem. 20: 4. PMID: 30961528
- A novel assay for triglycerides using glycerol dehydrogenase and a water-soluble formazan dye, WST-8. | Kawano, M., et al. 2019. Ann Clin Biochem. 56: 442-449. PMID: 30971104
- The integrity and stability of specimens under different storage conditions for glucose-6-phosphate dehydrogenase deficiency screening using WST-8. | Chamchoy, K., et al. 2021. Acta Trop. 217: 105864. PMID: 33607062
- Water spinach and okra sprouts inhibit cancer cell proliferation. | Matsuo, T., et al. 2022. In Vitro Cell Dev Biol Anim. 58: 79-84. PMID: 35132541
- A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet. | Ishiyama, M., et al. 1996. Biol Pharm Bull. 19: 1518-20. PMID: 8951178
Inhibitor of:
TGaseZ.Substrate of:
Chromogenic.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WST-8, 100 mg | sc-391198 | 100 mg | $286.00 | |||
WST-8, 500 mg | sc-391198A | 500 mg | $1020.00 | |||
WST-8, 1 g | sc-391198B | 1 g | $1615.00 | |||
WST-8, 5 g | sc-391198C | 5 g | $4650.00 | |||
WST-8, 10 g | sc-391198D | 10 g | $7680.00 |