WST-8 CAS: 193149-74-5
MF: C20H13N6NaO11S2
MW: 600.47
A tetrazolium reagent that serves as a chromogenic indicator for NADH.

WST-8 (CAS 193149-74-5)

WST-8 | CAS 193149-74-5 is rated 5.0 out of 5 by 1.
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Alternate Names: 2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium Sodium Salt; 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazolium, inner salt, monosodium salt
Application: WST-8 is a tetrazolium reagent that serves as a chromogenic indicator for NADH
CAS Number: 193149-74-5
Purity: ≥95%
Molecular Weight: 600.47
Molecular Formula: C20H13N6NaO11S2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nM. Useful as a cell viability indicator in cell proliferation assays.


References

1. Ishiyama, M. et al. 1996. Biol. Pharm. Bull. 19(11): 1518-1520. PMID: 8951178

2. Ishiyama, M. et al. 1997. Talanta. 44(7): 1299-1305. PMID: 18966866

3. Ukeda, H. et al. 1999. Biosci. Biotechnol. Biochem. 63(3): 485-488. PMID: 27393255

Physical State :
Solid
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
9894947
MDL Number :
MFCD09264687
SMILES :
COC1=C(C=CC(=C1)[N+](=O)[O-])[N+]2=NC(=NN2C3=CC=C(C=C3)[N+](=O)[O-])C4=C(C=C(C=C4)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+]

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Certificate of Analysis

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Does anyone know the molar absorptivity of WST-8

Asked by: AJK123
Thank you for your question. We could not locate the molar absorptivity, however, the following publications might be helpful. PMID: 8951178 PMID: 18966866 PMID: 27393255
Answered by: Technical Support
Date published: 2017-10-13

how to prepare the stock solution for cell viability assay?

Asked by: JennyLiu
Thank you for your question. We do not have a specific protocol for stock solutions for cell viability assays. A general stock solution ma be made by dissolving the WST-8 in the solvent of choice. WST-8 is soluble in the organic solvent DMSO, which should be purged with an inert gas. The solubility of WST-8 in DMSO is approximately 1 mg/ml. Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. Organic solvent-free aqueous solutions of WST-8 can be prepared by directly dissolving the crystalline solid in aqueous buffers. The solubility of WST-8 in PBS, pH 7.2, is approximately 5 mg/ml. We do not recommend storing the aqueous solution for more than one day.
Answered by: Technical Service
Date published: 2018-04-11

What is the solubility in DMSO?

Asked by: chemicalsmg
Thank you for your question. WST-8: sc-391198 is soluble in DMSO at approximately 1mg/ml.
Answered by: Chemical Support 3
Date published: 2017-03-24

What is WST-8 soluble in?

Asked by: SCchem16
Thank you for your question. This chemical is soluble in large polar solvents like H2O, DMSO, etc. It is not very soluble in ethyl acetate, etc. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2017-01-14
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Rated 5 out of 5 by from Various publications cite the use of WST Various publications cite the use of WST-8 as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nM. Useful as a cell viability indicator in cell proliferation assays. -SCBT Publication Review
Date published: 2015-01-23
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