Wnt-8a Antibody (14-3) recommended for detection of Wnt-8a of mouse origin by WB and ELISA

Wnt-8a Antibody (14-3): sc-80459

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Wnt-8a Antibody (14-3) is rated 2.0 out of 5 by 1.
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Datasheets

    • Anti-Wnt-8a Antibody (14-3) is a rat monoclonal IgG2a Wnt-8a antibody provided at 100 µg/ml
    • raised against full length recombinant Wnt-8a of mouse origin
    • recommended for detection of Wnt-8a of mouse origin by WB and ELISA; non cross-reactive with Wnt-1, Wnt-3a, Wnt-5a or Wnt-10b

How much sample do you recommend loading on the gel for Western blot detection using Wnt-8a (14-3): sc-80459?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-27
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