Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Wip1 Double Nickase Plasmid (h): sc-400980-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Wip1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Wip1 Double Nickase Plasmid (h) and Wip1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PPM1D. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Wip1 Antibody (F-10): sc-376257
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Wip1 Double Nickase Plasmid (h) | sc-400980-NIC | 20 µg | $410.00 | |||
Wip1 Double Nickase Plasmid (h2) | sc-400980-NIC-2 | 20 µg | $410.00 |
PPM1D encodes Wip1 (PPM1D), a Mg2+/Mn2+-dependent Ser/Thr protein phosphatase that acts as a feedback regulator of DNA damage signaling. Wip1 attenuates stress-response pathways by dephosphorylating key mediators including p53, ATM/ATR pathway components, and MAPK signaling nodes, thereby modulating cell-cycle checkpoint recovery and apoptosis. Through these activities, PPM1D influences genome stability, replication stress tolerance, and inflammatory signaling outputs. Altered PPM1D/Wip1 activity has been associated with dysregulated DNA repair and checkpoint control in multiple disease-relevant contexts, supporting its use as a mechanistic target in genome integrity research.
Wip1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PPM1D locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PPM1D. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PPM1D function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PPM1D-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.