WIBG CRISPR/Cas9 KO Plasmid (h): sc-409500
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WIBG CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the WIBG genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WIBG CRISPR/Cas9 KO Plasmid (h) | sc-409500 | 20 µg | $397.00 |
Overview
PYM1 encodes the human protein WIBG, a component of messenger ribonucleoprotein biology implicated in coordinating post-transcriptional gene regulation. WIBG has been linked to RNA metabolism processes that influence transcript stability and translation, supporting dynamic control of protein output in proliferating and differentiating cells. Through these roles, WIBG intersects with pathways governing proteome homeostasis and cellular stress responses that are frequently perturbed in disease-relevant contexts. Dysregulation of mRNA processing and translation is associated with diverse pathological states, making WIBG a useful target for mechanistic studies of gene expression control.
WIBG CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PYM1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PYM1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PYM1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish WIBG protein expression.
This CRISPR knockout system enables efficient generation of PYM1-deficient cell models for investigation of WIBG signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PYM1 exon(s) critical for WIBG function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PYM1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by WIBG CRISPR/Cas9 KO Plasmid (h) and WIBG CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PYM1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by WIBG HDR Plasmid (h) and WIBG HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PYM1 homology arms to support homology-directed repair at defined PYM1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.