Whirlin Antibody (A-12): sc-271508

Whirlin Antibody (A-12) is a mouse monoclonal IgG2b antibody that detects Whirlin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Whirlin is a crucial cytoplasmic protein containing PDZ domains, primarily localized in the cochlear hair cells of the inner ear, where Whirlin plays a vital role in the elongation and maintenance of stereocilia, the mechanosensory organelles essential for hearing. The proper localization of Whirlin ensures the effective assembly and function of stereocilia, which are necessary for converting sound vibrations into neural signals. Whirlin interacts with actin filaments and is part of a dynamic protein interactome associated with Usher syndrome, linking Whirlin to both auditory and visual functions. Notably, Whirlin′s interaction with Myosin XVa, a motor protein, facilitates Whirlin′s transport to the tips of stereocilia, promoting their elongation. Additionally, Whirlin′s interaction with p55 is critical, as mutations in the DFNB31 gene, which encodes Whirlin, can lead to early loss of p55 labeling in stereocilia, potentially resulting in recessive hearing loss in both rats and humans. Anti-Whirlin antibody (A-12) is an essential tool for researchers studying the molecular mechanisms underlying sensory organ function and related disorders.

The preferred Whirlin antibody is Whirlin Antibody (G-8): sc-365250.