Western Blotting Luminol Reagent
  • Santa Cruz Biotechnology Luminol reagent is a non-radioactive, light-emitting system for detecting proteins on a Western blot membrane. Horseradish peroxidase mediates oxidation of Luminol reagent in the presence of hydrogen peroxide and this reaction produces an iridescent light.
  • Western blotting Luminol reagent is recommended for Western blotting enhanced chemiluminescence applications using Horseradish Peroxidase (HRP) conjugated secondary antibodies.
  • One Western blotting Luminol reagent package contains 125 ml each Solution A and Solution B, which combine to form sufficient chemiluminescence reagent for a 2,000 cm squared membrane area.
  • Extended exposure times (multiple exposures) to film made possible by long-duration signal
  • Western blotting Luminol reagent solutions are stable at room temperature for one hour. Store at 2-8° C for longer periods up to 12 months. Store in the dark.

Western Blotting Luminol Reagent: sc-2048

Western Blotting Luminol Reagent is rated 4.6 out of 5 by 64.
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Datasheets

    • Santa Cruz Biotechnology Luminol reagent is a non-radioactive, light-emitting system for detecting proteins on a Western blot membrane. Horseradish peroxidase mediates oxidation of Luminol reagent in the presence of hydrogen peroxide and this reaction produces an iridescent light.
    • Western blotting Luminol reagent is recommended for Western blotting enhanced chemiluminescence applications using Horseradish Peroxidase (HRP) conjugated secondary antibodies.
    • One Western blotting Luminol reagent package contains 125 ml each Solution A and Solution B, which combine to form sufficient chemiluminescence reagent for a 2,000 cm squared membrane area.
    • Extended exposure times (multiple exposures) to film made possible by long-duration signal
    • Western blotting Luminol reagent solutions are stable at room temperature for one hour. Store at 2-8° C for longer periods up to 12 months. Store in the dark.
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Is Western Blotting Luminol Reagent: sc-2048 Can used membrane labeling with secondary antibody (Gout anti-rabbit-HRP)

Asked by: Dalal
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2022-05-25

Can you please tell the components of solution A and solution B?

Asked by: Prat
Thank you for your question. You can find the product's datasheet at the top of its product page.
Answered by: Technical Support Europe
Date published: 2022-05-25

May I ask you what is the composition of reagent B and what are the exact concentrations of the components, I was not able to find that thank you

Asked by: Nina12345
Thank you for your question. The composition of reagents A and B are considered proprietary. If you have any specific questions or concerns, please contact our Technical Service Department and we will do our best to assist you without revealing any proprietary information.
Answered by: Technical Support
Date published: 2022-05-25

Same problem here! the protocol is still not available on the website and did not arrive with the luminol! Please send it to me asap!

Asked by: Rina
Thank you for your question. The protocol for Western Blotting Luminol Reagent sc-2048 can be found in the datasheet. The datasheet is available from the product page or under the following link: https://datasheets.scbt.com/sc-2048.pdf
Answered by: SCBT Heidelberg Support
Date published: 2022-05-25

Would you please tell me how much time it needs to be in the membrane before imaging? 2 min? 5 min?

Asked by: mnopqr
Thank you for your question. We recommend using 0,125 ml per square centimeter of the membrane and incubating the membtane for 1 minute at room temperature. You can find the datasheet with the procedure for this product by following the link below: https://datasheets.scbt.com/sc-2048.pdf
Answered by: SCBT Heidelberg Support
Date published: 2022-05-25

Is it compatible only with mouse secondary? How about rabbit and other?

Asked by: Sireesh
Than you for your question. Our Western Blotting Luminol Reagent: sc-2048 is compatible with any Horseradish Peroxidase (HRP) conjugated secondary antibodies. The host or target species does not make any difference.
Answered by: Technical Support
Date published: 2022-05-25

The user guide on your website is a safety datasheet.I haven't found other guidelines.So if you're convenient, please send me one.I couldn't find mine anywhere.

Asked by: Huai
thank you very much for bringing this to our attention. We will update the corresponding link on our website shortly. I just sent you the technical datasheet with protocol be email.
Answered by: Technical Support Europe
Date published: 2018-08-28

volevo sapere che sensibilità ha questo ECL..fantogram o pico grazie

Asked by: alessandro
Grazie mille per la sua domanda, contatti pure il nostro supporto tecnico via email o telefono per avere informazioni più specifiche sul prodotto.
Answered by: Technical Support Europe
Date published: 2017-11-22
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Rated 5 out of 5 by from SCBT luminol Works extremely well, 1mL enough to cover mini blot
Date published: 2022-08-17
Rated 5 out of 5 by from Excellent Perfect reagent! Works very well with all types of secondary antibodies in WB. I have been using the sc-2048 for seven years.
Date published: 2022-07-22
Rated 5 out of 5 by from Works perfect Very good product. Easy to use. We have been using SantaCruz Western Blotting Luminol Reagent for a while and never had any problem. Excellent reagent, very stable at 4C.
Date published: 2022-03-29
Rated 5 out of 5 by from Great Good signal with less background. It also lasts for more time.
Date published: 2022-02-20
Rated 5 out of 5 by from Excellent I'd like to say so many thanks to this Western Blotting Luminol reagent. It is such an excellent product that our lab have used it for more than 10 years. And I'll use it in my following study life as ever.
Date published: 2021-08-23
Rated 5 out of 5 by from 反应灵敏 I bought this product a year ago. It is very functional and easy to use.
Date published: 2021-07-09
Rated 5 out of 5 by from 很优秀的底物 一个月前购买的底物,曝光效果很好,而且底物作用时间很长,爆出的片子也不脏,实验室的其他同学都说特别好用
Date published: 2021-07-07
Rated 4 out of 5 by from Very good Very good product. I used it for more than three years
Date published: 2021-01-04
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