WDR24 CRISPR/Cas9 KO Plasmid (h): sc-407847
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WDR24 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the WDR24 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDR24 CRISPR/Cas9 KO Plasmid (h) | sc-407847 | 20 µg | $397.00 |
Overview
WDR24 encodes a WD repeat–containing protein that functions as a core component of the GATOR2 complex, a key regulator of amino acid–sensing control of mTORC1 signaling. Through modulation of GATOR1 activity, WDR24 helps coordinate nutrient availability with lysosomal mTORC1 activation, impacting autophagy, protein synthesis, and cellular growth programs. Perturbation of this axis can alter metabolic homeostasis and stress responses, linking WDR24-associated pathways to mechanisms frequently studied in cancer metabolism, neurobiology, and other contexts where mTORC1 dysregulation is implicated. WDR24 is therefore relevant for dissecting nutrient signaling circuitry and its downstream transcriptional and translational outputs.
WDR24 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the WDR24 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the WDR24 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the WDR24 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish WDR24 protein expression.
This CRISPR knockout system enables efficient generation of WDR24-deficient cell models for investigation of WDR24 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting WDR24 exon(s) critical for WDR24 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple WDR24 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by WDR24 CRISPR/Cas9 KO Plasmid (h) and WDR24 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the WDR24 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by WDR24 HDR Plasmid (h) and WDR24 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by WDR24 homology arms to support homology-directed repair at defined WDR24 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.