WDR18 Double Nickase Plasmid (m): sc-432062-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WDR18 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- WDR18 Double Nickase Plasmid (m) and WDR18 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Wdr18. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDR18 Double Nickase Plasmid (m) | sc-432062-NIC | 20 µg | $410.00 | |||
WDR18 Double Nickase Plasmid (m2) | sc-432062-NIC-2 | 20 µg | $410.00 |
Wdr18 encodes WD repeat domain 18 (WDR18), a conserved WD40-repeat protein that functions as a core component of the small subunit (SSU) processome required for 18S rRNA maturation and 40S ribosome biogenesis. By supporting pre-rRNA processing and assembly of ribonucleoprotein complexes in the nucleolus, WDR18 helps maintain translational capacity and proteostasis during cell growth and proliferation. Disruption of SSU processome factors is broadly linked to nucleolar stress and altered cell-cycle control, making Wdr18 a relevant target for studying how ribosome biogenesis interfaces with stress signaling and developmental programs. In mouse systems, perturbing Wdr18 provides a handle to probe mechanisms connecting rRNA processing defects with phenotypes that resemble ribosomopathy-like cellular dysfunction.
WDR18 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Wdr18 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Wdr18. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Wdr18 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Wdr18-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.