VPS13A Double Nickase Plasmid (h): sc-407168-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VPS13A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- VPS13A Double Nickase Plasmid (h) and VPS13A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting VPS13A. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VPS13A Double Nickase Plasmid (h) | sc-407168-NIC | 20 µg | $410.00 | |||
VPS13A Double Nickase Plasmid (h2) | sc-407168-NIC-2 | 20 µg | $410.00 |
VPS13A encodes a large peripheral membrane protein that localizes at organelle contact sites and supports lipid transport and membrane homeostasis, with established roles in endosomal trafficking, autophagy, and mitochondrial function. In neurons and other metabolically active cells, VPS13A contributes to maintaining organelle morphology and intracellular distribution by coordinating lipid exchange and membrane remodeling events. Disruption of VPS13A perturbs cellular stress responses and vesicle dynamics, making it a key node for studying membrane contact site biology and organelle quality control pathways. Loss-of-function variants are linked to neurodegenerative phenotypes, providing a disease-relevant framework for mechanistic studies in human cell models.
VPS13A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the VPS13A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within VPS13A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt VPS13A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of VPS13A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.