VpreB3 Antibody (FT-32): sc-135605

VpreB3 Antibody (FT-32) is a mouse monoclonal IgG1 kappa light chain antibody that detects VpreB3 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-VpreB3 antibody (FT-32) is available as a non-conjugated format. VpreB3, also known as CD179a or pre-B lymphocyte 1, plays a crucial role in the early stages of B cell development, specifically in pre-B lymphocytes, where VpreB3 is expressed but absent in mature B cells and other blood cell lineages. VpreB3 is essential for forming a molecular complex with the immunoglobulin mu chain, which is vital for regulating immunoglobulin gene rearrangements during B cell differentiation. VpreB3 gene is located on human chromosome 22q11.2, and VpreB3 works in conjunction with the lambda 5 gene to produce the iota and omega polypeptide chains, respectively. In humans, these genes are separate entities, unlike in mice, where VpreB3 and lambda 5 are co-expressed as part of the same transcription unit. Proper functioning of VpreB3 is critical for the development of a functional immune response, as VpreB3 ensures the correct assembly of the B cell receptor complex, which is necessary for the maturation and activation of B cells.

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VpreB3 Antibody (FT-32) References:

1. VPREB3: cDNA characterization and expression in human and chromosome mapping in human and mouse.  |  Rosnet, O., et al. 1999. Cytogenet Cell Genet. 87: 205-8. PMID: 10702669
2. The VpreB protein of the surrogate light-chain can pair with some mu heavy-chains in the absence of the lambda 5 protein.  |  Seidl, T., et al. 2001. Eur J Immunol. 31: 1999-2006. PMID: 11449352
3. Analysis of VpreB expression during B lineage differentiation in lambda5-deficient mice.  |  Stephan, RP., et al. 2001. J Immunol. 167: 3734-9. PMID: 11564789
4. The VpreB1 enhancer drives developmental stage-specific gene expression in vivo.  |  Licence, S., et al. 2003. Eur J Immunol. 33: 1117-26. PMID: 12672078
5. Variable domains and a VpreB-like molecule are present in a jawless vertebrate.  |  Cannon, JP., et al. 2005. Immunogenetics. 56: 924-9. PMID: 15650874
6. The pre-B-cell receptor induces silencing of VpreB and lambda5 transcription.  |  Parker, MJ., et al. 2005. EMBO J. 24: 3895-905. PMID: 16281060
7. The human pre-B-specific lambda-like cluster is located in the 22q11.2-22q12.3 region, distal to the IgC lambda locus.  |  Mattei, MG., et al. 1991. Genomics. 9: 544-6. PMID: 1903358
8. The pre-B-cell receptor associated protein VpreB3 is a useful diagnostic marker for identifying c-MYC translocated lymphomas.  |  Rodig, SJ., et al. 2010. Haematologica. 95: 2056-62. PMID: 20823132
9. Pre-B Lymphocyte Protein 3 (VPREB3) Expression in the Adrenal Cortex: Precedent for non-Immunological Roles in Normal and Neoplastic Human Tissues.  |  Felizola, SJ., et al. 2015. Endocr Pathol. 26: 119-28. PMID: 25861052
10. A second gene, VpreB in the lambda 5 locus of the mouse, which appears to be selectively expressed in pre-B lymphocytes.  |  Kudo, A. and Melchers, F. 1987. EMBO J. 6: 2267-72. PMID: 3117530
11. The omega and iota surrogate immunoglobulin light chains.  |  Pillai, S. and Baltimore, D. 1988. Curr Top Microbiol Immunol. 137: 136-9. PMID: 3138073
12. The human Vpre B gene is located on chromosome 22 near a cluster of V lambda gene segments.  |  Bauer, SR., et al. 1988. Immunogenetics. 28: 328-33. PMID: 3139558