VMP1 Double Nickase Plasmid (h): sc-405940-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VMP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- VMP1 Double Nickase Plasmid (h) and VMP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting VMP1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VMP1 Double Nickase Plasmid (h) | sc-405940-NIC | 20 µg | $410.00 | |||
VMP1 Double Nickase Plasmid (h2) | sc-405940-NIC-2 | 20 µg | $410.00 |
VMP1 (vacuole membrane protein 1) is an integral membrane protein that supports autophagy initiation and autophagosome biogenesis, with prominent roles in phagophore formation and membrane remodeling at organelle contact sites. It coordinates lipid mobilization and organelle dynamics that influence ER–autophagosome communication, cellular stress adaptation, and intracellular quality control pathways. Through its impact on autophagic flux, VMP1 affects proteostasis, mitochondrial homeostasis, and inflammatory signaling outputs that shape cell survival and metabolic state. Altered VMP1 regulation has been investigated in contexts involving dysregulated autophagy, including tumor biology and neurodegeneration-associated stress responses, making it a useful node for pathway interrogation.
VMP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the VMP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within VMP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt VMP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of VMP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.