VILIP-1 Antibody (2F1-E3): sc-293209

VILIP-1 Antibody (2F1-E3) is a mouse monoclonal IgG2a kappa light chain antibody that detects VILIP-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-VILIP-1 antibody (2F1-E3) is available as a non-conjugated format. VILIP-1 protein, also known as visinin-like protein 1, is encoded by the VSNL1 gene located on the short arm of chromosome 2 and is a member of the neuronal calcium sensor family, which is part of the larger calcium-binding protein superfamily. VILIP-1 contains four EF-hand motifs and a double-stranded RNA-binding domain, which are crucial for calcium signaling. VILIP-1 is predominantly expressed in the membrane, cytoplasm, and cytoskeleton of sympathetic and parasympathetic neurons throughout the brain, with notable exceptions in the caudate-putamen region. Expression levels significantly decline with age, which may have implications for neuronal function and health. VILIP-1 interacts with actin in the cytoskeleton, facilitating translocation to the membrane, and binds to the 3′-untranslated region of trkB double-stranded mRNA in a calcium-dependent manner, highlighting a role in modulating neuronal signaling pathways. VILIP-1 associates with G protein-receptor kinase 1, inhibiting membrane binding, which suggests a regulatory function in G-protein signaling cascades. Decreased levels of VILIP-1 have been observed in the brains of individuals with Alzheimer′s disease, indicating potential involvement in the pathophysiology of this condition.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

VILIP-1 Antibody (2F1-E3) References:

1. Visinin-like protein (VILIP) is a neuron-specific calcium-dependent double-stranded RNA-binding protein.  |  Mathisen, PM., et al. 1999. J Biol Chem. 274: 31571-6. PMID: 10531361
2. Expression of the neuronal calcium sensor protein family in the rat brain.  |  Paterlini, M., et al. 2000. Neuroscience. 99: 205-16. PMID: 10938426
3. Regulation of G protein-coupled receptor kinase subtypes by calcium sensor proteins.  |  Sallese, M., et al. 2000. Biochim Biophys Acta. 1498: 112-21. PMID: 11108955
4. Decreased neurocalcin immunoreactivity in sympathetic and parasympathetic neurons of the major pelvic ganglion in aged rats.  |  Corns, RA., et al. 2001. Neurosci Lett. 297: 81-4. PMID: 11121875
5. Abnormal localization of two neuronal calcium sensor proteins, visinin-like proteins (vilips)-1 and -3, in neocortical brain areas of Alzheimer disease patients.  |  Braunewell, K., et al. 2001. Dement Geriatr Cogn Disord. 12: 110-6. PMID: 11173883
6. A meta-analysis on the levels of VILIP-1 in the CSF of Alzheimer's disease compared to normal controls and other neurodegenerative conditions.  |  Mavroudis, IA., et al. 2021. Aging Clin Exp Res. 33: 265-272. PMID: 31939203
7. Neurogranin and VILIP-1 as Molecular Indicators of Neurodegeneration in Alzheimer's Disease: A Systematic Review and Meta-Analysis.  |  Dulewicz, M., et al. 2020. Int J Mol Sci. 21: PMID: 33172069
8. Certification of visinin-like protein-1 (VILIP-1) certified reference material by amino acid-based and sulfur-based liquid chromatography isotope dilution mass spectrometry.  |  Zang, Y., et al. 2023. Anal Bioanal Chem. 415: 211-220. PMID: 36342508
9. Relationships of serum VILIP-1, NSE, and ADP levels with postoperative cognitive dysfunction in elderly patients undergoing general anesthesia: a retrospective, observational study.  |  Xie, Y. and Yao, Z. 2023. J Int Med Res. 51: 3000605231172447. PMID: 37194201
10. Cation binding and conformational changes in VILIP and NCS-1, two neuron-specific calcium-binding proteins.  |  Cox, JA., et al. 1994. J Biol Chem. 269: 32807-13. PMID: 7806504
11. Sequence characterization and genetic mapping of the human VSNL1 gene, a homologue of the rat visinin-like peptide RNVP1.  |  Polymeropoulos, MH., et al. 1995. Genomics. 29: 273-5. PMID: 8530085
12. The neuronal EF-hand Ca(2+)-binding protein VILIP: interaction with cell membrane and actin-based cytoskeleton.  |  Lenz, SE., et al. 1996. Biochem Biophys Res Commun. 225: 1078-83. PMID: 8780737

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-VILIP-1 Antibody (2F1-E3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-VILIP-1 Antibody (2F1-E3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

VILIP-1 Antibody (2F1-E3) Specifications

Alternate Product Name: Visinin Like 1

Product Clone Name: 2F1-E3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-191

Epitope Target: VILIP-1

Epitope Species: human

Product Reactivity/Detection: VILIP-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for VILIP-1 Antibody (2F1-E3): N/A