VCPIP1 Antibody (C-12): sc-515291

VCPIP1 Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects VCPIP1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VCPIP1 (C-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VCPIP1, also known as DUBA3 or VCIP135, is a 1,222 amino acid protein characterized by the presence of one OTU domain, and is primarily localized to the endoplasmic reticulum and golgi stacks within the golgi apparatus. This localization enables VCPIP1 to interact with valosin-containing protein (VCP) and p47, functioning as a deubiquitinating enzyme essential for post-mitotic golgi stack formation. VCPIP1 may also play a significant role in VCP-mediated creation of the transitional endoplasmic reticulum, which is vital for maintaining cellular homeostasis and proper protein processing. VCPIP1 is encoded on human chromosome 8, a region associated with various diseases and malignancies, including schizophrenia, bipolar disorder, and certain leukemias, highlighting VCPIP1′s importance in both cellular function and disease pathology.

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VCPIP1 Antibody (C-12) References:

1. VCIP135, a novel essential factor for p97/p47-mediated membrane fusion, is required for Golgi and ER assembly in vivo.  |  Uchiyama, K., et al. 2002. J Cell Biol. 159: 855-66. PMID: 12473691
2. Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation.  |  Ficarro, S., et al. 2003. J Biol Chem. 278: 11579-89. PMID: 12509440
3. VCIP135 acts as a deubiquitinating enzyme during p97-p47-mediated reassembly of mitotic Golgi fragments.  |  Wang, Y., et al. 2004. J Cell Biol. 164: 973-8. PMID: 15037600
4. p97/p47-Mediated biogenesis of Golgi and ER.  |  Uchiyama, K. and Kondo, H. 2005. J Biochem. 137: 115-9. PMID: 15749824
5. NSF/SNAPs and p97/p47/VCIP135 are sequentially required for cell cycle-dependent reformation of the ER network.  |  Kano, F., et al. 2005. Genes Cells. 10: 989-99. PMID: 16164599
6. p37 is a p97 adaptor required for Golgi and ER biogenesis in interphase and at the end of mitosis.  |  Uchiyama, K., et al. 2006. Dev Cell. 11: 803-16. PMID: 17141156
7. Hepatitis B Virus X Protein Is Stabilized by the Deubiquitinating Enzyme VCPIP1 in a Ubiquitin-Independent Manner by Recruiting the 26S Proteasome Subunit PSMC3.  |  Wu, Q., et al. 2022. J Virol. 96: e0061122. PMID: 35695579
8. PTP4A2 promotes lysophagy by dephosphorylation of VCP/p97 at Tyr805.  |  Bai, Y., et al. 2023. Autophagy. 19: 1562-1581. PMID: 36300783
9. Accelerating inhibitor discovery for deubiquitinating enzymes.  |  Chan, WC., et al. 2023. Nat Commun. 14: 686. PMID: 36754960
10. Molecular Basis of VCPIP1 and P97/VCP Interaction Reveals Its Functions in Post-Mitotic Golgi Reassembly.  |  Liao, T., et al. 2024. Adv Sci (Weinh). 11: e2403417. PMID: 39234822
11. Identification and characterization of host factor VCPIP1 as a multi-functional positive regulator of hepatitis B virus.  |  Kang, N., et al. 2024. J Virol. 98: e0158124. PMID: 39494904
12. VCPIP1 negatively regulates NF-κB signaling pathways by deubiquitinating and stabilizing Erbin in MDP-stimulated macrophages.  |  Zuo, J., et al. 2024. Int Immunopharmacol. 143: 113622. PMID: 39550842

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-VCPIP1 Antibody (C-12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-VCPIP1 Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

VCPIP1 Antibody (C-12) Specifications

Alternate Product Name: VCPIP1

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 84-293

Epitope Target: VCPIP1

Epitope Species: human

Product Reactivity/Detection: VCPIP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for VCPIP1 Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin