Vasorin CRISPR/Cas9 KO Plasmid (h): sc-404942
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Vasorin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Vasorin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Vasorin Antibody (4G7): sc-517034
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Vasorin CRISPR/Cas9 KO Plasmid (h) | sc-404942 | 20 µg | $397.00 |
Overview
VASN encodes vasorin, a type I transmembrane glycoprotein enriched in vascular smooth muscle cells that modulates extracellular signaling at the cell surface. Vasorin is reported to bind and sequester TGF-β ligands, influencing SMAD-dependent transcriptional programs that govern cell proliferation, migration, and extracellular matrix remodeling. Through these interactions, vasorin contributes to vascular homeostasis and tissue remodeling processes linked to atherosclerosis, vascular injury responses, and fibrosis-associated phenotypes. Altered VASN expression has also been observed in multiple tumor contexts, supporting its use in studies of microenvironmental signaling and cell-state transitions.
Vasorin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the VASN gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the VASN together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the VASN open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Vasorin protein expression.
This CRISPR knockout system enables efficient generation of VASN-deficient cell models for investigation of Vasorin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting VASN exon(s) critical for Vasorin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple VASN genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Vasorin CRISPR/Cas9 KO Plasmid (h) and Vasorin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the VASN locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Vasorin HDR Plasmid (h) and Vasorin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by VASN homology arms to support homology-directed repair at defined VASN target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.