VapG Antibody (F-3): sc-390681
- VapG Antibody (F-3) is a mouse monoclonal IgG2a κ VapG antibody provided at 200 µg/ml
- raised against amino acids 1-172 representing full length virulence associated protein virulence associated protein VapG of Rhodococcus equi origin
- VapG Antibody (F-3) is recommended for detection of virulence associated protein VapG of Rhodococcus equi origin by WB, IP, IF and ELISA
- Anti-VapG Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for VapG Antibody (F-3) for WB applications. These reagents are now offered in bundles with VapG Antibody (F-3) (see ordering information below).
QUICK LINKS
VapG Antibody (F-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects VapG protein of Rhodococcus equi origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VapG (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VapG plays a crucial role in the virulence of Rhodococcus equi, a gram-positive bacterium responsible for severe pyogranulomatous pneumonia in foals and immunocompromised humans, making VapG a significant concern in veterinary and human medicine. Understanding VapG structure is essential, as VapG is part of a family of virulence-associated proteins that facilitate the bacterium′s ability to evade the host immune response. VapG structure allows interaction with host immune cells, thereby modulating the immune response and contributing to the pathogenicity of R. equi. Infected foals typically mount an immune response against R. equi, with antibodies primarily targeting VapA, while responses to VapD, VapF, VapG, and VapH are observed at decreasing levels. VapG′s ability to elicit an immune response highlights VapG′s potential as a target for therapeutic interventions and vaccine development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VapG Antibody (F-3) References:
- DNA sequence and comparison of virulence plasmids from Rhodococcus equi ATCC 33701 and 103. | Takai, S., et al. 2000. Infect Immun. 68: 6840-7. PMID: 11083803
- Immunoglobulin G subisotype responses of pneumonic and healthy, exposed foals and adult horses to Rhodococcus equi virulence-associated proteins. | Hooper-McGrevy, KE., et al. 2003. Clin Diagn Lab Immunol. 10: 345-51. PMID: 12738629
- Deletion of vapA encoding Virulence Associated Protein A attenuates the intracellular actinomycete Rhodococcus equi. | Jain, S., et al. 2003. Mol Microbiol. 50: 115-28. PMID: 14507368
- Rhodococcus equi secreted antigens are immunogenic and stimulate a type 1 recall response in the lungs of horses immune to R. equi infection. | Kohler, AK., et al. 2003. Infect Immun. 71: 6329-37. PMID: 14573652
- The LysR-type transcriptional regulator VirR is required for expression of the virulence gene vapA of Rhodococcus equi ATCC 33701. | Russell, DA., et al. 2004. J Bacteriol. 186: 5576-84. PMID: 15317761
- VapI, a new member of the Rhodococcus equi Vap family. | Polidori, M. and Haas, A. 2006. Antonie Van Leeuwenhoek. 90: 299-304. PMID: 16871422
- Molecular characterization of Rhodococcus equi from horse-breeding farms by means of multiplex PCR for the vap gene family. | Monego, F., et al. 2009. Curr Microbiol. 58: 399-403. PMID: 19205798
- Development of a live, attenuated, potential vaccine strain of R. equi expressing vapA and the virR operon, and virulence assessment in the mouse. | Whitehead, AE., et al. 2012. Vet Immunol Immunopathol. 145: 479-84. PMID: 22088674
- Development of ELISA test for determination of the level of antibodies against Rhodococcus equi in equine serum and colostrum. | Witkowski, L., et al. 2012. Vet Immunol Immunopathol. 149: 280-5. PMID: 22883938
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VapG Antibody (F-3) | sc-390681 | 200 µg/ml | $322.00 | |||
VapG Antibody (F-3): m-IgG Fc BP-HRP Bundle | sc-530224 | 200 µg Ab; 10 µg BP | $361.00 | |||
VapG Antibody (F-3): m-IgGκ BP-HRP Bundle | sc-523634 | 200 µg Ab, 40 µg BP | $361.00 | |||
VapG Antibody (F-3): m-IgG2a BP-HRP Bundle | sc-547461 | 200 µg Ab; 10 µg BP | $361.00 | |||
VapG Antibody (F-3) AC | sc-390681 AC | 500 µg/ml, 25% agarose | $424.00 | |||
VapG Antibody (F-3) HRP | sc-390681 HRP | 200 µg/ml | $322.00 | |||
VapG Antibody (F-3) FITC | sc-390681 FITC | 200 µg/ml | $336.00 | |||
VapG Antibody (F-3) PE | sc-390681 PE | 200 µg/ml | $349.00 | |||
VapG Antibody (F-3) Alexa Fluor® 488 | sc-390681 AF488 | 200 µg/ml | $364.00 | |||
VapG Antibody (F-3) Alexa Fluor® 546 | sc-390681 AF546 | 200 µg/ml | $364.00 | |||
VapG Antibody (F-3) Alexa Fluor® 594 | sc-390681 AF594 | 200 µg/ml | $364.00 | |||
VapG Antibody (F-3) Alexa Fluor® 647 | sc-390681 AF647 | 200 µg/ml | $364.00 | |||
VapG Antibody (F-3) Alexa Fluor® 680 | sc-390681 AF680 | 200 µg/ml | $364.00 | |||
VapG Antibody (F-3) Alexa Fluor® 790 | sc-390681 AF790 | 200 µg/ml | $364.00 |