V5-Probe Antibody (C-9) recommended for detection of V5 fusion proteins by WB, IP, IF and ELISA

V5-Probe Antibody (C-9): sc-271944

V5-Probe Antibody (C-9) is rated 4.5 out of 5 by 8.
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Datasheets

    • V5-Probe Antibody (C-9) is a mouse monoclonal IgG2a κ, cited in 16 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 87-111 within a C-terminal sequence of the P and V proteins of Simian Virus 5 origin
    • V5-Probe Antibody (C-9) is recommended for detection of V5 fusion proteins by WB, IP, IF and ELISA
    • Anti-V5-Probe Antibody (C-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of V5-Probe (C-9): sc-271944.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for V5-Probe Antibody (C-9) for WB applications. This reagent is now offered in a bundle with V5-Probe Antibody (C-9) (see ordering information below).

is this antibody compatible with ChiP? The discontinued product was, but ChiP is NOT listed and application here and that it what I need it for.

Asked by: MonicaN
Thank you for your question. This mouse monoclonal antibody does not yet have data or product citations using it for ChIP, so it is not a recommended application at this time. We would recommend one of our alternative antibodies, V5-Probe Antibody (E10): sc-81594 , which has 2 known product citations using the antibody for ChIP. These references are on PubMed, PMID's: 29249655 and 20855289.
Answered by: Technical Support
Date published: 2020-03-26

Hello, I would like to know if the "GKPIPNPLLGLDST" is the immunogen used in the production of this antibody? Thanks in advance

Asked by: GUIGO31
Thank you for your question. The exact immunogen sequence is considered proprietary. Please contact Technical Service or your local distributor for information further information.
Answered by: Tech Service
Date published: 2019-03-20

What is the best secondary to use with V5-Probe Antibody (C-9): sc-271944 in Western Blot?

Asked by: cjMara
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-09
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Rated 5 out of 5 by from Nice and Clear Great antibody to probe expression in various cells using pLX304 vector for expression of Cterm v5-tagged protein. No troubleshooting, worked just fine at 1:10,000 dilution primary and 1:50,000 secondary using 50ug total protein loading per well (0.2um PVDF transfer using various MW proteins)
Date published: 2019-01-29
Rated 5 out of 5 by from Complete Successful completion of the target, parsing some proteins including transmembrane and secreted, were labeled and detected by immunofluorescence by Western blotting.
Date published: 2017-02-10
Rated 5 out of 5 by from Strong western Very clear, specific WB data; strong signal with little or no background
Date published: 2017-01-26
Rated 5 out of 5 by from Reliable Antibody, Great for WB This antibody is well-cited for a variety of applications including WB and ELISA. Strong, clear signal with little to no background. Very reliable antibody.
Date published: 2017-01-06
Rated 3 out of 5 by from Worked well in IP but not WB Worked well in IP but not WB.
Date published: 2015-10-29
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2015-06-01
Rated 4 out of 5 by from Publishable data in WB using human PC3 cell lines Publishable data in WB using human PC3 cell lines. -SCBT Publication Review
Date published: 2015-03-08
Rated 5 out of 5 by from IP and WB publishable data with HEK293T IP and WB publishable data with HEK293T and HeLa cell line - SCBT Publication Review
Date published: 2014-12-08
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