Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Uteroglobin/SCGB1A1/CC10 Double Nickase Plasmid (h): sc-400381-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Uteroglobin/SCGB1A1/CC10 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Uteroglobin/SCGB1A1/CC10 Double Nickase Plasmid (h) and Uteroglobin/SCGB1A1/CC10 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SCGB1A1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Uteroglobin/SCGB1A1/CC10 Antibody (E-11): sc-365992
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Uteroglobin/SCGB1A1/CC10 Double Nickase Plasmid (h) | sc-400381-NIC | 20 µg | $410.00 | |||
Uteroglobin/SCGB1A1/CC10 Double Nickase Plasmid (h2) | sc-400381-NIC-2 | 20 µg | $410.00 |
SCGB1A1 encodes uteroglobin (also known as CC10/CCSP), a secreted member of the secretoglobin family predominantly produced by airway club cells and other mucosal epithelia. Uteroglobin functions as an anti-inflammatory, immunomodulatory, and antioxidant-associated protein that can influence epithelial barrier homeostasis and regulate responses to environmental injury, in part by modulating cytokine signaling and eicosanoid-related processes. SCGB1A1 expression is widely used as a marker of club cell differentiation and airway epithelial state, linking it to pathways governing mucosal defense, tissue repair, and innate immune regulation. Altered SCGB1A1 levels and club cell dysfunction are studied in the context of inflammatory airway conditions, smoke or pollutant exposure responses, and epithelial remodeling phenotypes relevant to respiratory disease models.
Uteroglobin/SCGB1A1/CC10 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SCGB1A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SCGB1A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SCGB1A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SCGB1A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.