USP18 Double Nickase Plasmid (m): sc-423988-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP18 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- USP18 Double Nickase Plasmid (m) and USP18 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Usp18. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP18 Double Nickase Plasmid (m) | sc-423988-NIC | 20 µg | $410.00 |
Mouse Usp18 encodes ubiquitin specific peptidase 18 (USP18), an ISG15-specific deconjugating enzyme that also functions as a key negative regulator of type I interferon signaling. USP18 attenuates JAK–STAT pathway output by limiting IFNAR2-associated signaling and helps shape antiviral and inflammatory transcriptional programs downstream of interferon-stimulated genes. Through control of ISGylation and cytokine responsiveness, USP18 influences innate immune homeostasis, myeloid cell activation, and cellular stress adaptation. Dysregulated USP18 activity has been linked to aberrant interferon responses relevant to autoinflammation, infection biology, and tumor-immune interactions, making Usp18 a useful locus for mechanistic studies of interferon-driven phenotypes in mouse models.
USP18 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Usp18 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Usp18. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Usp18 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Usp18-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.