URB Double Nickase Plasmid (h): sc-408597-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- URB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- URB Double Nickase Plasmid (h) and URB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CCDC80. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
URB Double Nickase Plasmid (h) | sc-408597-NIC | 20 µg | $410.00 | |||
URB Double Nickase Plasmid (h2) | sc-408597-NIC-2 | 20 µg | $410.00 |
CCDC80 encodes URB, a secreted extracellular matrix–associated protein implicated in stromal remodeling, cell adhesion, and regulation of cell migration. URB has been linked to signaling networks that influence adipogenesis and metabolic homeostasis, including crosstalk with TGF-β–related pathways and extracellular matrix organization. Altered CCDC80 expression has been reported across multiple tumor types and in metabolic and fibrotic contexts, supporting its use as a molecular node for studying microenvironmental regulation of proliferation, invasion, and differentiation. These properties make URB a relevant target for dissecting gene–matrix interactions and transcriptional programs controlling tissue remodeling.
URB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CCDC80 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CCDC80. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CCDC80 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CCDC80-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.