UPIIIb Double Nickase Plasmid (h): sc-407617-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UPIIIb Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UPIIIb Double Nickase Plasmid (h) and UPIIIb Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UPK3B. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UPIIIb Double Nickase Plasmid (h) | sc-407617-NIC | 20 µg | $410.00 | |||
UPIIIb Double Nickase Plasmid (h2) | sc-407617-NIC-2 | 20 µg | $410.00 |
UPK3B encodes uroplakin IIIb (UPIIIb), a tetraspanin-like membrane protein that contributes to the specialized apical surface architecture of urothelial cells. UPIIIb participates in uroplakin complex assembly and trafficking to the asymmetric unit membrane, supporting barrier integrity and membrane differentiation programs in the urinary tract epithelium. Altered UPK3B expression has been reported across contexts of epithelial remodeling and urothelial lineage dysregulation, making it a useful molecular handle for studying differentiation state and membrane organization. In human cell models, UPK3B perturbation is commonly evaluated alongside pathways controlling cell junctions, cytoskeletal coupling, and vesicular transport at the apical membrane.
UPIIIb Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UPK3B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UPK3B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UPK3B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UPK3B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.