Unc18-1 Antibody (31): sc-136304

Unc18-1 Antibody (31) is a mouse monoclonal IgG1 antibody that detects Unc18-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Unc18-1 antibody (31) is available as a non-conjugated format. Unc18-1 protein plays a crucial role in regulating exocytosis by acting as a chaperone that prevents premature interactions between syntaxin and soluble NSF attachment protein receptors, which are essential for vesicle fusion and neurotransmitter release. This regulation is vital for maintaining proper cellular communication and function, particularly in neurons and endocrine cells. Additionally, Unc18-1 undergoes post-translational modifications, including phosphorylation at specific sites such as Ser 313 and Thr 574, which modulate interactions with Syntaxin 1A and influence exocytotic processes. Unc18-1 mRNA expression in various cell types, including RBL-2H3 mast cells and mouse bone marrow-derived mast cells, underscores its importance in immune responses and cellular signaling pathways. Unc18-1 (31) monoclonal antibody serves as an essential tool for researchers studying exocytosis mechanisms and SNARE protein roles in cellular communication.

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Unc18-1 Antibody (31) References:

1. Phosphorylation of Munc18 by protein kinase C regulates the kinetics of exocytosis.  |  Barclay, JW., et al. 2003. J Biol Chem. 278: 10538-45. PMID: 12519779
2. A role for Sec1/Munc18 proteins in platelet exocytosis.  |  Schraw, TD., et al. 2003. Biochem J. 374: 207-17. PMID: 12773094
3. Alcoholic chronic pancreatitis involves displacement of Munc18c from the pancreatic acinar basal membrane surface.  |  Gaisano, HY., et al. 2004. Pancreas. 28: 395-400. PMID: 15097857
4. Syntaxin/Munc18 interactions in the late events during vesicle fusion and release in exocytosis.  |  Graham, ME., et al. 2004. J Biol Chem. 279: 32751-60. PMID: 15175344
5. Regulation of syntaxin1A-munc18 complex for SNARE pairing in HEK293 cells.  |  Gladycheva, SE., et al. 2004. J Physiol. 558: 857-71. PMID: 15218059
6. Fluorescence resonance energy transfer reports properties of syntaxin1a interaction with Munc18-1 in vivo.  |  Liu, J., et al. 2004. J Biol Chem. 279: 55924-36. PMID: 15489225
7. Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions.  |  Ciufo, LF., et al. 2005. Mol Biol Cell. 16: 470-82. PMID: 15563604
8. Protease-activated receptor-1 activation of endothelial cells induces protein kinase Calpha-dependent phosphorylation of syntaxin 4 and Munc18c: role in signaling p-selectin expression.  |  Fu, J., et al. 2005. J Biol Chem. 280: 3178-84. PMID: 15576373
9. Expression and transcriptional regulation of Munc18 isoforms in mast cells.  |  Nigam, R., et al. 2005. Biochim Biophys Acta. 1728: 77-83. PMID: 15777698
10. STXBP1 as a therapeutic target for epileptic encephalopathy.  |  Stamberger, H., et al. 2017. Expert Opin Ther Targets. 21: 1027-1036. PMID: 28971703
11. Delineating clinical and developmental outcomes in STXBP1-related disorders.  |  Xian, J., et al. 2023. Brain. 146: 5182-5197. PMID: 38015929
12. Accelerating therapeutic development and clinical trial readiness for STXBP1 and SYNGAP1 disorders.  |  Marotta, N., et al. 2024. Curr Probl Pediatr Adolesc Health Care. 54: 101576. PMID: 38472035

The preferred Unc18-1/2 antibody is Unc18-1/2 Antibody (D-1): sc-133169.