UltraCruz^® Protease Inhibitor Cocktail Tablet

To use UltraCruz^® Protease Inhibitor Cocktail Tablets, simply dissolve 1 tablet in 50 ml of buffer or lysate. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents. UltraCruz^® Protease Inhibitor Cocktail Tablets do not contain phosphatase inhibitors. If necessary, tablets can be added along with the protease inhibitor tablets.

To use UltraCruz^® Protease Inhibitor Cocktail Tablets, simply dissolve 1 tablet in 50 ml of buffer or lysate. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents. UltraCruz^® Protease Inhibitor Cocktail Tablets do not contain phosphatase inhibitors. If necessary, Phosphatase Inhibitor Cocktail tablets can be added along with the protease inhibitor tablets.

Note: Solution may be slightly hazy when tablet is dissolved.



Additional Notes:


• Each tablet contains protease inhibitors sufficient for 50 ml cell extract. If very high proteolytic activity is present, one tablet should be used for 25 ml extraction buffer.

• 25x Stock Solution: Dissolve one tablet in 2 ml milliQ H2O or in 100 mM phosphate buffer, pH 7.0.

• Tablet contains EDTA (18.5 mg/tablet yielding 1 mM solution of EDTA in 50 ml); the extraction buffer should not contain divalent cations such as Ca²⁺, Mg²⁺ or Mn^2-.

• Also Available: UltraCruz^® Protease Inhibitor Cocktail Tablet, EDTA-free (sc-29131).


Stability:

• The tablets are stable at +2 to +8° C, stored dry.

• Tablets may discolor if stored for extended periods at room temperature. The color change does not affect inhibitor activity.

• The stock solution is stable for 1–2 weeks, stored at +2 to +8° C, or for 12 weeks at -15 to -25° C.