UltraCruz® Protease Inhibitor Cocktail Tablet A mixture of reversible and irreversible protease inhibitors.

UltraCruz® Protease Inhibitor Cocktail Tablet

UltraCruz Protease Inhibitor Cocktail Tablet is rated 5.0 out of 5 by 3.
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Alternate Names: UltraCruz® Protease Inhibitor Cocktail Tablet is also known as Protease Inhibitor Tablet
Application: UltraCruz® Protease Inhibitor Cocktail Tablet is a mixture of reversible and irreversible protease inhibitors
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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UltraCruzTM Protease Inhibitor Cocktail Tablet contains a mixture broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments. For the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts. UltraCruzTM Protease Inhibitor Cocktail Tablets contains both reversible and irreversible protease inhibitors.
UltraCruzTM Protease Inhibitor Cocktail Tablets protect proteins from proteolytic degradation through inhibition of the serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. UltraCruzTM Protease Inhibitor Cocktail Tablets contain AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A and are available in formulations with or without EDTA, a metalloproteinase inhibitor. The EDTA-free formulation (sc-29131) has added compatibility with IMAC purification methods and 2D gel electrophoresis. UltraCruzTM Protease Inhibitor Cocktail Tablets are stable for storage at 4°C for up to 12 months, each tablet is sufficient for 50mL of solution. Suitable for reagent preparation prior to protein extraction from tissue and cultured cells.


References

1. Qin, S., et al. 2006. Invest. Ophthalmol. Vis. Sci. 47: 5098-5105. PMID: 17065531

2. Singleton, K.D. and Wischmeyer, P.E. 2006. Shock. 25: 295-299. PMID: 16552363

3. Gohil, K., et al. 2008. Brain Res. 1201: 167-176. PMID: 18299118

4. Qin, S. and De Vries, G.W. 2008. J. Biol. Chem. 283: 6744-6751. PMID: 18195011

5. Poloz, Y.O. and O′Day, D.H. 2009. Int. J. Legal Med. 123: 305-314. PMID: 19326139

Usage :
To use UltraCruzTM Protease Inhibitor Cocktail Tablets, simply dissolve 1 tablet in 50mL of buffer or lysate. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents. UltraCruzTM Protease Inhibitor Cocktail Tablets do not contain phosphatase inhibitors. If necessary, Phosphatase Inhibitor Cocktail tablets can be added along with the protease inhibitor tablets.

Note: Solution may be slightly hazy when tablet is dissolved.



Additional Notes:


• Each tablet contains protease inhibitors sufficient for 50 ml cell extract. If very high proteolytic activity is present, one tablet should be used for 25 ml extraction buffer.

• 25x Stock Solution: Dissolve one tablet in 2 ml milliQ H2O or in 100 mM phosphate buffer, pH 7.0.

• Tablet contains EDTA (18.5 mg/tablet yielding 1 mM solution of EDTA in 50 ml); the extraction buffer should not contain divalent cations such as Ca2+, Mg2+ or Mn2-.

Also Available: UltraCruzTM Protease Inhibitor Cocktail Tablet, EDTA-free (sc-29131).


Stability:

• The tablets are stable at +2 to +8°C, stored dry.

• Tablets may discolor if stored for extended periods at room temperature. The color change does not affect inhibitor activity.

• The stock solution is stable for 1–2 weeks, stored at +2 to +8°C, or for 12 weeks at -15 to -25°C.
Appearance :
Tablet
Physical State :
Solid
Storage :
Desiccate at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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UltraCruz® Protease Inhibitor Cocktail Tablet  Product Citations

See how others have used UltraCruz® Protease Inhibitor Cocktail Tablet. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 78 total

PMID: # 34067817  Coimbra-Costa, D.|Garzón, F.|Alva, N.|Pinto, TCC.|Aguado, F.|Torrella, JR.|Carbonell, T.|Rama, R.| et al. 2021. Int J Mol Sci. 22:

PMID: # 33806619  Algarín, EM.|Quwaider, D.|Campos-Laborie, FJ.|Díaz-Tejedor, A.|Mogollón, P.|Vuelta, E.|Martín-Sánchez, M.|San-Segundo, L.|González-Méndez, L.|Gutiérrez, NC.|García-Sanz, R.|Paíno, T.|De Las Rivas, J.|Ocio, EM.|Garayoa, M.| et al. 2021. Cells. 10:

PMID: # 34584194  Tran, QH.|Hoang, DH.|Song, M.|Choe, W.|Kang, I.|Kim, SS.|Ha, J.| et al. 2021. Exp Mol Med. 53: 1413-1422.

PMID: # 34638792  Zaripova, KA.|Kalashnikova, EP.|Belova, SP.|Kostrominova, TY.|Shenkman, BS.|Nemirovskaya, TL.| et al. 2021. Int J Mol Sci. 22:

PMID: # 33654787  Misiewicz-Krzeminska, I.|Isidro, I.|Gutiérrez, NC.| et al. 2019. Bio Protoc. 9: e3267.

PMID: # 31046517  Sharlo, K.|Paramonova, I.|Turtikova, O.|Tyganov, S.|Shenkman, B.| et al. 2019. J. Appl. Physiol. 126: 1769-1781.

PMID: # 29253460  Fang, K. et al. 2018. Am. J. Pathol. 188: 586-599.

PMID: # 28839114  Belova, SP. et al. 2017. Physiol Rep. 5:

PMID: # 28776759  Wang, CK.|Ahmed, MM.|Jiang, Q.|Lu, NN.|Tan, C.|Gao, YP.|Mahmood, Q.|Chen, DY.|Fukunaga, K.|Li, M.|Chen, Z.|Wilcox, CS.|Lu, YM.|Qin, ZH.|Han, F.| et al. 2017. J. Pineal Res. 63:

PMID: # 27131125  McCombs, JE. et al. 2016. Carbohydrate research. 428: 31-40.

Citations 1 to 10 of 78 total

For how long is the solution stable after the tablets are dissolved?

Asked by: SCM4
Stock solution remains stable for 1–2 weeks when refrigerated (+2°C to +8°C), or up to 12 weeks when frozen (-15°C to -25°C).
Answered by: Tech Service 11
Date published: 2017-02-22
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Rated 5 out of 5 by from Really good quality product Really good quality product.
Date published: 2015-09-11
Rated 5 out of 5 by from Qin Qin, et. al. (PubMed ID 17065531) successfully utilized product sc-29130 in their cell extraction reagent. -SCBT Publication Review
Date published: 2015-05-20
Rated 5 out of 5 by from Various publications utilized product sc Various publications utilized product sc-29310 in lysis reagents to prevent protein degradation. -SCBT Publication Review
Date published: 2015-05-20
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