Date published: 2025-9-28

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Nitrocellulose Membranes, Unsupported – UltraCruz®

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  • Nitrocellulose pure transfer membrane is an unsupported nitrocellulose membrane that is most effective for protein or immunoblotting applications.
  • Nitrocellulose pure transfer membrane is cellulose acetate free, assuring high binding and sensitivity with low backgrounds. Nucleic acid binding is 100 µg/cm2.
  • 0.22 µm pore size nitrocellulose membrane is recommended for peptides and low molecular weight proteins (<13,000 Daltons). 0.45 µm pore size nitrocellulose membrane is recommended for higher molecular proteins (13 kDa-400 kDa).
  • Nitrocellulose membrane is easily blocked.
  • Nitrocellulose membrane is compatible with isotopic, chemiluminescent luminol, colorimetric, and fluorescence imaging.
  • Applications: Western Blot, Immunoblot, Southern Blot, Northern Blot, Dot Blot, Radiolabelled, chromogenic and chemiluminescent detection systems
  • sc-201705, sc-201706: Manufactured by GVS.
  • DISCOUNT: Bulk orders of any combination of Lab Supplies totaling:
    • $500 or more receive a 5% discount
    • $1,500 or more receive a 10% discount
    • $2,500 or more receive a 15% discount

FREE GROUND SHIPPING
on labware orders over $20 in the contiguous US

Description
UltraCruz® Nitrocellulose Membranes, Unsupported are recommended for nucleic acid and immunoblotting assays, Western blot, dot blot, chromogenic and chemiluminescent detection systems. Unsupported nitrocellulose membrane is made of 100% pure nitrocellulose and is free of cellulose acetate ensuring highest binding capacity and sensitivity with low background for optimal protein, peptide, DNA or RNA analysis. Pure nitrocellulose membrane with 0.22 µm pore size is ideal for electrophoretic transfer of low molecular weight proteins that are less than 13 kilodaltons or peptides and nucleic acids with less than 300 base pairs while the more commonly used membrane with pore size of 0.45 µm is recommended for transferring proteins with molecular weight between 13 kDa - 400 kDa or peptides and nucleic acids with greater than 300 bp. Nitrocellulose Pure Transfer Membranes are compatible with isotopic, chemiluminescent luminol, colorimetric, and fluorescence imaging. Unsupported nitrocellulose membrane is not suitable stripping and reprobing applications.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

UltraCruz® Nitrocellulose Pure Transfer Membrane, 0.22 µm, 30cmx3m roll

sc-3718
1 roll
$315.95

UltraCruz® Nitrocellulose Pure Transfer Membrane, 0.45µm, 15cmx3m roll

sc-201706
1 roll
$185.95

UltraCruz® Nitrocellulose Pure Transfer Membrane, 0.45µm, 20cmx3m roll

sc-201705
1 roll
$225.95

UltraCruz® Nitrocellulose Pure Transfer Membrane, 0.45 µm, 30 cm x 3 m roll

sc-3724
1 roll
$345.95

Hi, what side should be in contact with the acrylamide gel? the porous side or the more plastic side? thank you.

Asked by: Taby
Thank you for your question. The more porous side should be facing the gel.
Answered by: Tech Service
Date published: 2025-03-11

Which are good transfer conditions with this membrane for big proteins around 460 kDA?

Asked by: Annii
Thank you for your question. As this question has a length answer, please contact our technical service for assistance, we are happy to help you with your transfer condition question.
Answered by: Tech Service
Date published: 2019-02-22

Good morning all, Do you have the MSDS for this product? UltraCruz&reg; Nitrocellulose Pure Transfer Membranes

Asked by: Johanna
Thank you for your question. No, we do not provide an MSDS/SDS for this product. Please contact our Technical Service for further support.
Answered by: Technical Support
Date published: 2019-02-02

With regards to fluorescence would the membrane also allow the green channel to be viewed (Alexa Fluor 488)?

Asked by: Aslam
Thank you for your question. This membrane also supports direct or indirect detection with fluorochrome-conjugated antibodies. Please contact our technical service if you require further information.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06
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Rated 5 out of 5 by from Great transfer membraneWe use the 0,45 um variant and works great with most of the antibodies we have tested.
Date published: 2022-07-15
Rated 5 out of 5 by from Excellent membranes for Western blot!We have been using this product for several years and it has been working consistently great.
Date published: 2020-01-11
Rated 5 out of 5 by from Perfect!I bought this NC, got good results. Clear background.
Date published: 2018-01-26
Rated 4 out of 5 by from Great QualityI have been using this membrane from Santa Cruz for a while and the quality is excellent.
Date published: 2018-01-17
Rated 5 out of 5 by from very excellent product!The whole lab would love to use it for their western blot!
Date published: 2017-09-02
Rated 4 out of 5 by from great features!I bought this many times and am happy with the results.
Date published: 2017-06-08
Rated 5 out of 5 by from nitrocellulosethese membranes performed exceptionally well for westerns
Date published: 2017-04-05
Rated 5 out of 5 by from Fantastic product!I bought this membrane in December and have been using it for the past month and it works really well.
Date published: 2017-02-06
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Nitrocellulose Membranes, Unsupported – UltraCruz ® is rated 4.7 out of 5 by 19.
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