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UltraCruz® Hard-set Mounting Medium: sc-516213

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Datasheets
  • For mounting and preservation of fluorescence-stained tissues and cells
  • Hardens when slides are cover-slipped preventing need for additional adhesive like nail polish or plastic sealant
  • Provides superior anti-fade and anti-photobleaching properties similar to UltraCruz® Aqueous Mounting Medium (sc-24941)
  • Can be used for in situ hybridization techniques or for other procedures requiring fluorescent labeling of DNA
  • Supplied as a 10 ml aqueous solution
  • Inhibits photobleaching of probes containing fluorescein, Texas Red, Rhodamine and other fluorochromes including CruzFluor™ (CFL) fluorescent dyes
  • For a hard-set mounting medium that contains DAPI (sc-3598) for DNA counterstaining, we would recommend our alternative product, UltraCruz® Hard-set Mounting Medium with DAPI (sc-359850)
  • Store mounted slides at 4° C protected from light
  • Stable for 6 months from date of purchase
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UltraCruz® Hard-set Mounting Medium is an essential tool in microscopy and histological analysis, designed to preserve and protect microscopic samples on slides. This mounting medium is particularly valued for its hard-setting properties, which, upon drying, create a durable, clear encapsulation that enhances the optical qualities necessary for detailed imaging. The medium functions by matching the refractive index of glass, significantly reducing light scattering and improving the clarity and contrast of the visualized sample. Its solvent-based formulation initiates a curing process when exposed to air, leading to solvent evaporation and the formation of a hard polymer that secures the tissue or cellular structures to the slide. This stabilization is crucial not only for maintaining the physical integrity of the sample during long-term storage but also for preserving the spatial arrangement of biomolecules, especially in applications involving fluorescent labeling. By preventing the diffusion of fluorophores, UltraCruz® Hard-set Mounting Medium ensures that the fluorescent signals remain sharp and localized, facilitating precise analysis in research studies focused on cellular morphology and molecular localization, such as immunofluorescence and in situ hybridization, without any implications for clinical or therapeutic use.

UltraCruz® Hard-set Mounting Medium References:

  1. Long-term alterations of colonic nerve-mast cell interactions induced by neonatal maternal deprivation in rats.  |  Barreau, F., et al. 2008. Gut. 57: 582-90. PMID: 18194988
  2. Delivery of siRNA into the cytoplasm by liposomal bubbles and ultrasound.  |  Negishi, Y., et al. 2008. J Control Release. 132: 124-30. PMID: 18804499
  3. Processing of MIA protein during melanoma cell migration.  |  Schmidt, J. and Bosserhoff, AK. 2009. Int J Cancer. 125: 1587-94. PMID: 19521988
  4. Selection of an immunohistochemical panel for cardiovascular research in sheep.  |  De Visscher, G., et al. 2010. Appl Immunohistochem Mol Morphol. 18: 382-91. PMID: 20090513
  5. Expression of mitochondrial branched-chain aminotransferase and α-keto-acid dehydrogenase in rat brain: implications for neurotransmitter metabolism.  |  Cole, JT., et al. 2012. Front Neuroanat. 6: 18. PMID: 22654736
  6. Systematic mutagenesis of α-synuclein reveals distinct sequence requirements for physiological and pathological activities.  |  Burrε, J., et al. 2012. J Neurosci. 32: 15227-42. PMID: 23100443
  7. Cre-expressing neurons in the cortical white matter of Ntsr1-Cre GN220 mice.  |  Sundberg, SC. and Granseth, B. 2018. Neurosci Lett. 675: 36-40. PMID: 29580883
  8. Normal microscopic anatomy of equine body and limb skin: A morphological and immunohistochemical study.  |  Jørgensen, E., et al. 2018. Ann Anat. 218: 205-212. PMID: 29730469
  9. Time course and magnitude of alpha-synuclein inclusion formation and nigrostriatal degeneration in the rat model of synucleinopathy triggered by intrastriatal α-synuclein preformed fibrils.  |  Patterson, JR., et al. 2019. Neurobiol Dis. 130: 104525. PMID: 31276792
  10. Role of Projections between Piriform Cortex and Orbitofrontal Cortex in Relapse to Fentanyl Seeking after Palatable Food Choice-Induced Voluntary Abstinence.  |  Reiner, DJ., et al. 2020. J Neurosci. 40: 2485-2497. PMID: 32051327
  11. Microglia Regulate Cell Genesis in a Sex-dependent Manner in the Neonatal Hippocampus.  |  Nelson, LH., et al. 2021. Neuroscience. 453: 237-255. PMID: 33129890
  12. The Impact of Milk Fat Globule Membrane with Exercise on Age-Related Degeneration of Neuromuscular Junctions.  |  Sugita, S., et al. 2021. Nutrients. 13: PMID: 34371820
  13. Evaluation of Gene Sensitivity to Radiation and Heavy Metals  |  Hind, J; De Villiers, N. :November 2006. Epidemiology. 17(6): p S343-S344,.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

UltraCruz® Hard-set Mounting Medium

sc-516213
10 ml
$171.00

老板,你好,你把这款试剂的具体使用说明书发给我一下嘛!我想和你家其他几种功能相似的试剂对比一下,选择合适于我们实验的。

Asked by: 红雨随心
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

老板,你好,你把这款试剂的具体使用说明书发给我一下嘛!我想和你家其他几种功能相似对比一下

Asked by: 红雨随心
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Is it suitable for ICC ? is it colorless?

Asked by: RAAD
Thank you for your question. This is suitable for fluorescence-stained tissues and cells. Yes, it is colorless.
Answered by: Technical Support
Date published: 2019-02-10
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Rated 1 out of 5 by from Lump FormationI see a huge (70% of volume) lump inside the vial. Even upon keeping the same at Room temperature, the lump is still present. Is it how it will be, or it has gone wrong in my case.
Date published: 2019-07-10
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UltraCruz® Hard-set Mounting Medium is rated 1.0 out of 5 by 1.
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