Date published: 2026-4-20

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UltraCruz® Aqueous Mounting Medium with DAPI: sc-24941

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Datasheets
  • For mounting and preservation of fluorescence-stained tissues and cells
  • Provides superior anti-fade and anti-photobleaching properties
  • Can be used for in situ hybridization techniques or for other procedures requiring fluorescent labeling of DNA
  • Supplied as a 10 ml aqueous solution
  • Inhibits photobleaching of probes containing fluorescein, Texas Red, Rhodamine and other fluorochromes including CruzFluor™ (CFL) fluorescent dyes
  • Contains 1.5 µg/ml 4',6-diamidino-2-phenylindole (DAPI) (sc-3598) for DNA counterstaining. DAPI produces a blue fluorescence with ex = 360 nm and em = 460 nm when bound to DNA
  • Mounted sections retain fluorescence during prolonged storage if permanently sealed around perimeter with nail polish or a plastic sealant
  • Store mounted slides at 4° C protected from light
  • Stable for 6 months from date of purchase
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UltraCruz® Aqueous Mounting Medium with DAPI is an essential tool in fluorescence microscopy, designed for the specific purpose of staining and visualizing cell nuclei within biological specimens. This water-based medium includes DAPI (4′,6-diamidino-2-phenylindole), a fluorescent stain known for its affinity to DNA, particularly binding to adenine-thymine rich regions. When bound, DAPI emits a bright blue fluorescence under ultraviolet light, making it invaluable for clear and precise imaging of nuclear structures and other DNA-containing organelles. The aqueous nature of the medium ensures that water-soluble components of the specimen are preserved, preventing dehydration and maintaining the structural integrity of the cells for extended analysis. This feature is particularly beneficial for long-term storage of slides, allowing researchers to revisit and reanalyze samples without loss of data quality. Used widely in cell biology, genetics, and developmental biology research, UltraCruz® Aqueous Mounting Medium with DAPI facilitates detailed observation and documentation of cellular morphology and nuclear organization, providing insights into cellular function and architecture without the toxicity associated with some organic solvent-based mounting media. This makes it an indispensable resource in advanced scientific studies where detailed, non-destructive visualization of cell structures is required.

UltraCruz® Aqueous Mounting Medium with DAPI References:

  1. Hypertension exacerbates predisposition to neurodegeneration and memory impairment in the presence of a neuroinflammatory stimulus: Protection by angiotensin converting enzyme inhibition.  |  Goel, R., et al. 2015. Pharmacol Biochem Behav. 133: 132-45. PMID: 25869103
  2. Mechanisms of Retinal Damage after Ocular Alkali Burns.  |  Paschalis, EI., et al. 2017. Am J Pathol. 187: 1327-1342. PMID: 28412300
  3. CRISPR/Cas9Mediated Tspo Gene Mutations Lead to Reduced Mitochondrial Membrane Potential and Steroid Formation in MA-10 Mouse Tumor Leydig Cells.  |  Fan, J., et al. 2018. Endocrinology. 159: 1130-1146. PMID: 29300865
  4. Retinal degeneration rat model: A study on the structural and functional changes in the retina following injection of sodium iodate.  |  Koh, AE., et al. 2019. J Photochem Photobiol B. 196: 111514. PMID: 31154277
  5. Free light chains injure proximal tubule cells through the STAT1/HMGB1/TLR axis.  |  Upadhyay, R., et al. 2020. JCI Insight. 5: PMID: 32544092
  6. Fluorescence Colocalization Analysis of Cellular Distribution of MOR-1.  |  Borgonetti, V. and Galeotti, N. 2021. Methods Mol Biol. 2201: 27-34. PMID: 32975786
  7. 3D micro-organisation printing of mammalian cells to generate biological tissues.  |  Jeffries, GDM., et al. 2020. Sci Rep. 10: 19529. PMID: 33173097
  8. A novel method for isolation and culture of primary swine gastric epithelial cells.  |  Bautista-Amorocho, H., et al. 2021. BMC Mol Cell Biol. 22: 1. PMID: 33407092
  9. Insights Into the Mechanisms of Brain Endothelial Erythrophagocytosis.  |  Sun, J., et al. 2021. Front Cell Dev Biol. 9: 672009. PMID: 34409026
  10. Effects of amantadine and topiramate on neuronal damage in rats with experimental cerebral ischemia-reperfusion.  |  Gυndυz, ZB., et al. 2021. Adv Clin Exp Med. 30: 1013-1023. PMID: 34510842
  11. Distribution of α-synuclein in normal human jejunum and its relations with the chemosensory and neuroendocrine system.  |  Casini, A., et al. 2021. Eur J Histochem. 65: PMID: 34726359
  12. Resolvin D1 Suppresses H2O2-Induced Senescence in Fibroblasts by Inducing Autophagy through the miR-1299/ARG2/ARL1 Axis.  |  Kim, HJ., et al. 2021. Antioxidants (Basel). 10: PMID: 34943028
  13. Chitosan and Hyaluronic Acid Nanoparticles as Vehicles of Epoetin Beta for Subconjunctival Ocular Delivery.  |  Silva, B., et al. 2022. Mar Drugs. 20: PMID: 35200680
  14. Oviductal Oxygen Homeostasis in Patients with Uterine Myoma: Correlation between Hypoxia and Telocytes.  |  Wrona, A., et al. 2022. Int J Mol Sci. 23: PMID: 35682833

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

UltraCruz® Aqueous Mounting Medium with DAPI

sc-24941
10 ml
$196.00

Hi, I am conducting immunostaining experiments using UltraCruz® Aqueous Mounting Medium with DAPI: sc-24941. When I analyze my samples, I wanted to know what the refractive index is for the product because this helps determine the clarity of my images

Asked by: GotEm
Thank you for your question. The refractive index is 1.45.
Answered by: BlakeJ
Date published: 2023-08-02

hi.. i would like to ask . what is the different between sc-359850, sc-24941, and sc-3598? and is there any protocol to use these specific products? for instance the dilution ratio, solvent etc. i will use this together with sc-81648 AF488. Thankyou

Asked by: ccccc
For protocol and product inquiry Please contact Technical Service.
Answered by: Tech Sales
Date published: 2021-05-12

老板,你把这款产品的具体使用说明书发给我一下嘛!我想与你家的其他几种功能相似的试剂对比一下,选择合适于我们实验的。

Asked by: 红雨随心
Thanks for your question. Please check details on the link:https://www.scbt.com/p/ultracruz-aqueous-mounting-medium-with-dapi?requestFrom=search. If you have any other questions,please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website. 感谢您的提问。关于产品的详细信息详见链接:https://www.scbt.com/p/ultracruz-aqueous-mounting-medium-with-dapi?requestFrom=search。如有其他问题请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-11-14

Dear Sir/Madame, I would like to ask for quotation on UltraCruz® Mounting Medium Product Code SC-24941. Regards, Anna Anna Mietelska-Porowska, PhD Nencki Institute of Experimental Biology PAS, 3 Pasteur Street, 02-093 Warsaw, Poland

Asked by: annanencki
To receive a quote for a product, or to obtain accurate availability, shipping and pricing information for UltraCruz® Mounting Medium sc-24941, please contact our European Customer Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com. Alternatively, you can contact our local distributor in Poland, https://www.scbt.com/scbt/resources/distributors.
Answered by: Tech Service
Date published: 2017-02-10
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Rated 5 out of 5 by from ExcellentThis medium is excellent for mounting fluorescence stained sections or cells and also gives excellent staining for the nuclei. Two birds in one stone.
Date published: 2017-07-20
Rated 5 out of 5 by from very good stable reagent for IFgood Mounting Medium for IF staining, also very low price than othern supplier's .
Date published: 2017-05-28
Rated 5 out of 5 by from UltraCruz Mounting Mediumsc-24941, I used to biopsy, simple operation, long time for the preservation of the slide, good effect.
Date published: 2017-05-22
Rated 5 out of 5 by from good results.The product worked well in immunohistochemistry. We used it in the biopsy, simple operation, save time long, easy to save, and above all, we got good results.
Date published: 2017-02-09
Rated 5 out of 5 by from import impactThese DAPI-based UltraCruz water-based sealing media inhibit photobleaching of probes containing fluorescein, Texas Red, Rhodamine, and other fluorescent dyes including CruzFluor fluorescent dyes, which have a significant impact on the study process
Date published: 2017-02-05
Rated 5 out of 5 by from stableUsed product sc-24941, a medium used for preservation of fluorescence-stained tissue sections, with DAPI to analyze MCF-7 WT cells, It is very stable and we highly recommend this produ.
Date published: 2016-12-18
Rated 5 out of 5 by from Highly RecommendedWe are using and will continue using this product. It is very stable and we highly recommend this product.
Date published: 2016-12-01
Rated 5 out of 5 by from Very stableVery stable.
Date published: 2015-11-04
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UltraCruz® Aqueous Mounting Medium with DAPI is rated 5.0 out of 5 by 10.
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