UFD1 Antibody (E-4): sc-271221
- UFD1 Antibody (E-4) is a mouse monoclonal IgG3 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 294-337 at the C-terminus of UFD1 of human origin
- recommended for detection of UFD1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and porcine
- m-IgG3 BP-HRP is the preferred secondary detection reagent for UFD1 Antibody (E-4) for WB applications. This reagent is now offered in a bundle with UFD1 Antibody (E-4) (see ordering information below).
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UFD1 Antibody (E-4) is a mouse monoclonal IgG3 antibody that detects UFD1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UFD1 plays a crucial role in the ubiquitin-proteasome system, which is essential for maintaining cellular homeostasis by regulating protein degradation. UFD1 is primarily located in the cytoplasm and nucleus, where UFD1 participates in the recognition and processing of ubiquitin-conjugated substrates, thereby influencing the degradation of misfolded or damaged proteins. Proper functioning of UFD1 helps prevent the accumulation of potentially toxic proteins that could disrupt cellular functions. Notably, mutations in UFD1 have been linked to CATCH22 syndrome, a condition that manifests with various developmental defects, including cardiac anomalies and cleft palate, underscoring UFD1′s importance in normal development and cellular health. Anti-UFD1 antibody (E-4) serves as an invaluable tool for researchers studying protein degradation mechanisms and UFD1′s implications in disease.
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UFD1 Antibody (E-4) References:
- Role of the ubiquitin-selective CDC48(UFD1/NPL4)chaperone (segregase) in ERAD of OLE1 and other substrates. | Braun, S., et al. 2002. EMBO J. 21: 615-21. PMID: 11847109
- Involvement of the p97-Ufd1-Npl4 complex in the regulated endoplasmic reticulum-associated degradation of inositol 1,4,5-trisphosphate receptors. | Alzayady, KJ., et al. 2005. J Biol Chem. 280: 34530-7. PMID: 16103111
- Ufd1-Npl4 is a negative regulator of cholera toxin retrotranslocation. | McConnell, E., et al. 2007. Biochem Biophys Res Commun. 355: 1087-90. PMID: 17331469
- The p97 ATPase dislocates MHC class I heavy chain in US2-expressing cells via a Ufd1-Npl4-independent mechanism. | Soetandyo, N. and Ye, Y. 2010. J Biol Chem. 285: 32352-9. PMID: 20702414
- Concerted action of the ubiquitin-fusion degradation protein 1 (Ufd1) and Sumo-targeted ubiquitin ligases (STUbLs) in the DNA-damage response. | Køhler, JB., et al. 2013. PLoS One. 8: e80442. PMID: 24265825
- Structural Details of Ufd1 Binding to p97 and Their Functional Implications in ER-Associated Degradation. | Le, LT., et al. 2016. PLoS One. 11: e0163394. PMID: 27684549
- Structure of the Cdc48 ATPase with its ubiquitin-binding cofactor Ufd1-Npl4. | Bodnar, NO., et al. 2018. Nat Struct Mol Biol. 25: 616-622. PMID: 29967539
- PCBP2 Posttranscriptional Modifications Induce Breast Cancer Progression via Upregulation of UFD1 and NT5E. | Wang, X., et al. 2021. Mol Cancer Res. 19: 86-98. PMID: 33037085
- A non-symmetrical p97 conformation initiates a multistep recruitment of Ufd1/Npl4. | Arie, M., et al. 2024. iScience. 27: 110061. PMID: 38947518
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UFD1 Antibody (E-4) | sc-271221 | 200 µg/ml | $322.00 | |||
UFD1 Antibody (E-4): m-IgG3 BP-HRP Bundle | sc-550399 | 200 µg Ab; 40 µg BP | $361.00 | |||
UFD1 (E-4) Neutralizing Peptide | sc-271221 P | 100 µg/0.5 ml | $69.00 |