UDPase Antibody (D-4) recommended for detection of UDPase of and Streptococcus equi subsp. zooepidemicus/Streptococcus equi subsp. equi 4047 origin by WB, IP, IF and ELISA

UDPase Antibody (D-4): sc-398133

UDPase Antibody (D-4) is rated 5.0 out of 5 by 1.
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Datasheets

    • Anti-UDPase Antibody (D-4) is a mouse monoclonal IgG1 (kappa light chain) UDPase antibody provided at 200 µg/ml
    • raised against amino acids 1-300 representing full length UDPase of Streptococcus equi subsp. zooepidemicus origin
    • Anti-UDPase Antibody (D-4) is recommended for detection of UDPase of and Streptococcus equi subsp. zooepidemicus/Streptococcus equi subsp. equi 4047 origin by WB, IP, IF and ELISA
    • Anti-UDPase Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of UDPase (D-4): sc-398133.

How much sample do you recommend loading on the gel for Western blot detection using UDPase (D-4): sc-398133?

Asked by: Dr Ninau Qelp
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Very good Western blot data of UDPase expression Very good Western blot data of UDPase expression in Streptococcus equi subsp. zooepidemicus, Streptococcus equi (virulent), Streptococcus equi (avirulent), Rhodococcus equi and Escherichia coli whole cell lysates. -SCBT QC
Date published: 2013-07-03
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