UDP-GlcDH Antibody (A-9) recommended for detection of UDP-GlcDH of mouse, rat and human origin by WB, IP, IF and ELISA

UDP-GlcDH Antibody (A-9): sc-393438

UDP-GlcDH Antibody (A-9) is rated 5.0 out of 5 by 2.
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Datasheets

    • UDP-GlcDH Antibody (A-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 459-486 at the C-terminus of UDP-GlcDH of human origin
    • recommended for detection of UDP-GlcDH of mouse, rat and human origin by WB, IP, IF and ELISA
    • blocking peptide, sc-393438 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

What blotting conditions were used for the blot? dilution? blocking agent? I used the antibody at 1:1000 in 5% milk/TBST. It was almost clean enough but could be a little better.

Asked by: Joy N
Thank you for your question. You can find dilution recommendations for each antibody on their respective datasheets. Our protocol for western blotting that is used by our labs as well can be found under this link: https://www.scbt.com/resources/protocols/western-immuno-blotting We recommend UltraCruz® Blocking Reagent: sc-516214. Alternatively, a milk-based blocking buffer will produce a cleaner blot while BSA will provide stronger signals. Incubating the blot at a low dilution over night at 4 °C can also help to produce a robust and specific signal in many cases.
Answered by: Tech Support Europe
Date published: 2020-07-09

What application is the blocking peptide sc-393438 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclear Produced beautiful immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-23
Rated 5 out of 5 by from Nice Western Blot data of UDP Nice Western Blot data of UDP-GlcDH expression in Hep G2, HeLa and A-431 whole cell lysates and human liver tissue extract . -SCBT QC
Date published: 2014-04-23
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