Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
UDG Double Nickase Plasmid (h): sc-403189-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UDG Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UDG Double Nickase Plasmid (h) and UDG Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UNG. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UDG Antibody (B-7): sc-390255
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UDG Double Nickase Plasmid (h) | sc-403189-NIC | 20 µg | $410.00 | |||
UDG Double Nickase Plasmid (h2) | sc-403189-NIC-2 | 20 µg | $410.00 |
Human UNG encodes uracil-DNA glycosylase (UDG), a key initiator of base excision repair that excises uracil from DNA arising from cytosine deamination or dUTP misincorporation. By generating abasic sites that are subsequently processed by AP endonucleases, polymerases, and ligases, UNG helps maintain genome integrity during DNA replication and repair. UNG activity also intersects with antibody diversification processes in B cells by shaping the processing of uracil lesions introduced during somatic hypermutation and class switch recombination. Dysregulated uracil processing and impaired base excision repair have been linked to elevated mutational burden and genome instability phenotypes relevant to cancer biology and inflammation-associated DNA damage models.
UDG Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UNG locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UNG. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UNG function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UNG-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.