Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
UDG CRISPR/Cas9 KO Plasmid (h): sc-403189
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UDG CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UDG genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UDG Antibody (B-7): sc-390255
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UDG CRISPR/Cas9 KO Plasmid (h) | sc-403189 | 20 µg | $397.00 |
Overview
Human UNG encodes uracil-DNA glycosylase (UDG), a key base excision repair enzyme that initiates removal of uracil from DNA generated by cytosine deamination or misincorporation of dUMP during replication. By excising uracil and creating an abasic site for downstream processing, UNG helps preserve genome stability and limits mutagenesis in both nuclear and mitochondrial genomes. UNG activity interfaces with replication stress responses, DNA damage signaling, and pathways that counteract deamination-driven lesions, including processes relevant to antibody diversification and innate restriction of retroviral DNA. Dysregulation of uracil repair and associated repair intermediates has been linked to elevated mutation burden and genomic instability phenotypes studied across cancer biology, immunology, and host–pathogen interactions.
UDG CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the UNG gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the UNG together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the UNG open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UDG protein expression.
This CRISPR knockout system enables efficient generation of UNG-deficient cell models for investigation of UDG signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting UNG exon(s) critical for UDG function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple UNG genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UDG CRISPR/Cas9 KO Plasmid (h) and UDG CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the UNG locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UDG HDR Plasmid (h) and UDG HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by UNG homology arms to support homology-directed repair at defined UNG target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.