Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
UCP4 Double Nickase Plasmid (h): sc-417530-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UCP4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UCP4 Double Nickase Plasmid (h) and UCP4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC25A27. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UCP4 Antibody (A-5): sc-365295
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UCP4 Double Nickase Plasmid (h) | sc-417530-NIC | 20 µg | $410.00 | |||
UCP4 Double Nickase Plasmid (h2) | sc-417530-NIC-2 | 20 µg | $410.00 |
SLC25A27 encodes uncoupling protein 4 (UCP4), an inner mitochondrial membrane carrier that modulates proton leak and mitochondrial coupling efficiency, shaping oxidative phosphorylation output and reactive oxygen species homeostasis. UCP4 is enriched in neural tissues and is linked to regulation of mitochondrial membrane potential, calcium handling, and cellular stress responses that influence neuronal metabolism and survival. Through effects on mitochondrial bioenergetics, UCP4 intersects with pathways governing redox balance and programmed cell death, making it relevant to studies of neurodegeneration and metabolic vulnerability. Altered UCP4 expression or activity has been associated with disrupted energy homeostasis in disease-relevant contexts, supporting mechanistic investigation in human cell models.
UCP4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC25A27 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC25A27. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC25A27 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC25A27-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.