UCKL1 Antibody (B-11): sc-515466
- UCKL1 Antibody (B-11) is a mouse monoclonal IgG1 κ UCKL1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 31-142 mapping near the N-terminus of UCKL1 of human origin
- UCKL1 Antibody (B-11) is recommended for detection of UCKL1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-UCKL1 Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for UCKL1 Antibody (B-11) for WB applications. These reagents are now offered in bundles with UCKL1 Antibody (B-11) (see ordering information below).
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UCKL1 Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects UCKL1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UCKL1 (B-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UCKL1, also known as uridine-cytidine kinase 1-like 1, UCK1L, URKL1, or F538, is a 548 amino acid protein that plays a crucial role in pyrimidine metabolism, which is essential for DNA and RNA synthesis. UCKL1 is primarily localized in the cytoplasm but can translocate to the nucleus through interaction with the Epstein-Barr virus nuclear antigen 3A (EBNA-3A). Once in the nucleus, UCKL1 is involved in the ATP-dependent ribonucleotide salvage pathway, where UCKL1 catalyzes the conversion of uridine triphosphate (UTP) and cytidine triphosphate (CTP) into uridine monophosphate (UMP) and cytidine monophosphate (CMP), respectively. This function is vital for cell proliferation and the transformation of lymphoblasts, highlighting UCKL1′s importance in cellular growth and division. Additionally, UCKL1 is subject to ubiquitin-mediated degradation via the E3 ligase NKLAM, which regulates UCKL1 levels within the cell. The presence of three isoforms of UCKL1, resulting from alternative splicing, further underscores the complexity and regulatory mechanisms governing UCKL1′s function in cellular metabolism.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UCKL1 Antibody (B-11) References:
- Epstein-Barr virus encoded nuclear protein EBNA-3 binds a novel human uridine kinase/uracil phosphoribosyltransferase. | Kashuba, E., et al. 2002. BMC Cell Biol. 3: 23. PMID: 12199906
- NK lytic-associated molecule, involved in NK cytotoxic function, is an E3 ligase. | Fortier, JM. and Kornbluth, J. 2006. J Immunol. 176: 6454-63. PMID: 16709802
- Functional characterization of a gene encoding a dual domain for uridine kinase and uracil phosphoribosyltransferase in Arabidopsis thaliana. | Islam, MR., et al. 2007. Plant Mol Biol. 63: 465-77. PMID: 17143579
- Epstein-Barr virus nuclear proteins EBNA-3A and EBNA-3C are essential for B-lymphocyte growth transformation. | Tomkinson, B., et al. 1993. J Virol. 67: 2014-25. PMID: 8445720
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UCKL1 Antibody (B-11) | sc-515466 | 200 µg/ml | $322.00 | |||
UCKL1 Antibody (B-11): m-IgG Fc BP-HRP Bundle | sc-531382 | 200 µg Ab; 10 µg BP | $361.00 | |||
UCKL1 Antibody (B-11): m-IgGκ BP-HRP Bundle | sc-525058 | 200 µg Ab, 40 µg BP | $361.00 | |||
UCKL1 Antibody (B-11): m-IgG1 BP-HRP Bundle | sc-544516 | 200 µg Ab; 20 µg BP | $361.00 | |||
UCKL1 Antibody (B-11) AC | sc-515466 AC | 500 µg/ml, 25% agarose | $424.00 | |||
UCKL1 Antibody (B-11) HRP | sc-515466 HRP | 200 µg/ml | $322.00 | |||
UCKL1 Antibody (B-11) FITC | sc-515466 FITC | 200 µg/ml | $336.00 | |||
UCKL1 Antibody (B-11) PE | sc-515466 PE | 200 µg/ml | $349.00 | |||
UCKL1 Antibody (B-11) Alexa Fluor® 488 | sc-515466 AF488 | 200 µg/ml | $364.00 | |||
UCKL1 Antibody (B-11) Alexa Fluor® 546 | sc-515466 AF546 | 200 µg/ml | $364.00 | |||
UCKL1 Antibody (B-11) Alexa Fluor® 594 | sc-515466 AF594 | 200 µg/ml | $364.00 | |||
UCKL1 Antibody (B-11) Alexa Fluor® 647 | sc-515466 AF647 | 200 µg/ml | $364.00 | |||
UCKL1 Antibody (B-11) Alexa Fluor® 680 | sc-515466 AF680 | 200 µg/ml | $364.00 | |||
UCKL1 Antibody (B-11) Alexa Fluor® 790 | sc-515466 AF790 | 200 µg/ml | $364.00 |